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蛋白质/抗原/多肽/酶蛋白质/抗原/多肽/酶蛋白质/抗原/多肽/酶
Product Information GeneFist ?series3-color High Range Protein Marker(9-245 kDa)GF6619Size:250 μl × 15 μl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume? 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.? 1.5~2.5 μl per well for general Western blottingtransfer.? Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6619 GeneFist? 3-color High Range ProteinMarker is a ready to use three-color protein standardwith 12 pre-stained proteins covering a wide range ofmolecular weights from 10 to 245 kDa in Tris-Glycinebuffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins arecovalently coupled with different chromophores foreasy identification of bands, with two referenceproteins carrying enhanced intensity correspondingto a green at 25 kDa and red at 75 kDa, respectively,as separated on SDS-PAGE (Tris-Glycine buffer).The GF6619 GeneFist? 3-color High Range ProteinMarker is designed for monitoring protein separationduring SDS-polyacrylamide gel electrophoresis,verification of Western transfer efficiency onmembranes (PVDF, nylon, or nitrocellulose) and forapproximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6619 GeneFist? 3-color High Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue2452352352Blue1801701703Blue1401301304Blue10093935Red7570726Blue6053537Blue4541428Blue3530309Green25222310Blue20181811Blue15141412Blue109103-color High Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6619 GeneFist? 3-color High Range Protein Marker resolves 12 majorbands in SDS-PAGE (Tris-Glycine, MOPS, and MESbuffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 μl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 μl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 μl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 μl × 2GF6616 GeneFist 3-color Regular Range ProteinMarker, 250 μl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 μl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 μl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 μl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 μl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 μl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 μl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 μl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 μlGF5220 GeneFist 100 bp DNA Ladder II, 500 μlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 μlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 μlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 μlGeneFist Western Marker IFor research use only
Product Information GeneFist ? series3-color Regular Range Protein Marker(9-180 kDa)GF6616Size:250 μl x 15 μl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume? 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.? 1.5~2.5 μl per well for general Western blottingtransfer.? Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6616 GeneFist? 3-color Regular RangeProtein Marker is a ready to use three-color proteinstandard with 10 pre-stained proteins covering a widerange of molecular weights from 10 to 180 kDa (9 to170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDain Bis-Tris (MES) buffer). Proteins are covalentlycoupled with a blue chromophore except for tworeference bands (one green and one red band at 25kDa and 75 kDa respectively) when separated on SDSPAGE (Tris-Glycine buffer).The GF6616 GeneFist? 3-color Regular RangeProtein Marker is designed for monitoring proteinseparation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiencyon membranes, (PVDF, nylon, or nitrocellulose) andfor approximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6616 GeneFist? 3-color Regular Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue1801701702Blue1401301303Blue10093934Red7570725Blue6053536Blue4541427Blue3530308Green2522239Blue15141410Blue109103-color Regular Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6616 GeneFist?3-color Regular Range Protein Marker resolves 10major bands in SDS-PAGE (Tris-Glycine, MOPS, andMES buffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 μl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 μl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 μl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 μl × 2GF6616 GeneFist Enhanced 3-color Regular RangeProtein Marker, 250 μl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 μl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 μl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 μl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 μl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 μl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 μl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 μl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 μlGF5220 GeneFist 100 bp DNA Ladder II, 500 μlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 μlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 μlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 μlGeneFist Western Marker IFor research use only
Proteinase K from Tritirachium album | 蛋白酶K 来源于林伯氏白色念球菌lyophilized powder, >=30 units/mg protein, for molecular biology经验分子式（希尔表示法）|分子量|Beilstein Registry Number|EINECS 编号 254-457-8|产品特性摩尔质量： mol wt 28.93 kDa运输方式 ：湿冰运输外源活性位点 ：DNAse, Nickase and RNAse, none detected组成 ：Protein, >=95% biuret杂质 ：<=500 pg/mg DNA (PicoGreen(R) assay)级别 ：for molecular biology形态 ：冻干粉储存温度 ：-20°C
品牌：Merck/默克密理博 请电话或QQ联系客服询价！应用 应用Proteinase K (from Tritirachium album) chromatographically purified lyophilized 30 mAnson-U/mg for biochemistry and molecular biology EC 3.4.21.14. CAS 39450-01-6, pH 6.2 - 6.8 (10 g/l, H?O, 20 °C).理化信息密度1.1 g/cm3pH值6.2 - 6.8 (10 g/l, H?O, 20 °C)容积密度35 kg/m3规格Appearance (color)whiteAppearance (description)lyophilisateActivity (hemoglobin; pH?7.5; 37?°C)≥ 30.0 mAnsonU/mgSpec. activity (calc. on protein)≥ 40 mAnsonU/mgDNases (Nicking activity; pBR 322; 6?h; 37?°C)not detectableRNases (RNA; 2 h; 37°C)not detectable
品牌：Gibco描述This liquid formulation of trypsin contains EDTA and phenol red. Gibco Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco trypsin solutions and recommended dissociation conditions.This trypsin solution is modified as follows:With? EDTA? Phenol RedThe complete formulation is available.We offer a variety of trypsin formulations, including animal origin-free TrypLE reagents.Quality testingGibco Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, prior to manufacturing, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.Documented traceabilityWe can provide detailed documentation to meet your regulatory needs. Gibco Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.cGMP manufacturing and quality systemGibco Trypsin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.规格Chelators: EDTA分类分类: Animal OriginOsmolality: 270 - 320 mOsm/kgProduct Line: Gibco?试剂类型试剂类型: TrypsinShipping Condition: Wet Ice经过测试: In Vitro Bioassay性状: LiquidPhenol Red Indicator: Phenol RedpH 范围: 7.2 - 8.0浓度: 1 X数量: 100mL内容及储存Storage conditions: -5°C to -20°CShipping conditions: Wet iceShelf life: 24 months from date of manufacture
产品简介：胰酶细胞消化液(Trypsin-EDTA Solution)含 0.25%胰酶（Trypsin）和 0.02%EDTA，不含Ca2＋和 Mg2＋。该消化液已用 0.22 μm 滤膜过滤除菌，可以直接用于培养细胞的消化，或者一些组织的消化。本胰酶细胞消化液具有方便快速的特点，通常室温消化 1 分钟左右就可以消化下大多数贴壁细胞。使用说明：贴壁细胞的消化：1、吸去培养液，用无菌的 PBS、Hanks 液或无血清培养液洗涤细胞一次，以去除残余的血清。2、加入少量胰酶细胞消化液，略盖过细胞即可，室温放置 30 秒至 2 分钟。不同的细胞消化时间有所不同。3、显微镜下观察，细胞明显收缩，并且肉眼观察培养器皿底部发现细胞的形态发生明显的变化，或者用枪吹打细胞发现细胞刚好可以被吹打下来；此时吸除胰酶细胞消化液；加入含血清的完全细胞培养液，吹打下细胞，即可直接用于后续实验。4、如果发现消化不足，则加入胰酶细胞消化液重新消化。5、如果发现细胞消化时间过长，未及吹打细胞，细胞已经有部分直接从培养器皿底部脱落，直接用胰酶细胞培养液把细胞全部吹打下来。1000-2000g 离心 1 分钟，沉淀细胞，尽量去除胰酶细胞消化液后，加入含血清的完全培养液重新悬浮细胞，即可用于后续实验。 组织的消化：不同的组织需要消化的时间相差很大，通常以消化后可以充分打散组织为宜。注意事项：1、 在使用胰酶细胞消化液的过程中要特别注意避免消化液被细菌污染。2、 胰酶细胞消化液消化细胞时间不宜过长，否则细胞铺板后生长状况会较差。保存条件：4℃保存；长期贮存于-20℃中；-20℃贮存下保存一年。
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