中文 
bs-0162R，bioss，氧化氮合成酶-2（诱导型）抗体 iNOS
基本售价：980.00元
产品编号bs-0162R英文名称iNOS中文名称一氧化氮合成酶-2（诱导型）抗体别 名i NOS; Nitric Oxide Synthase, Inducible; HEP NOS; Hepatocyte NOS; HEPNOS; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; INOS; Inosl; MAC NOS; Macrophage NOS; Nitric oxide synthase 2 inducible macrophage; Nitric oxide synthase 2A (inducible hepatocytes); Nitric oxide synthase inducible; NOS 2; NOS 2A; NOS; Nos II; NOS type II; Nos2; NOS2A; NOS2_HUMAN. 研究领域细胞生物 免疫学 神经生物学 信号转导 转录调节因子 激酶和磷酸酶 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, Chicken, Dog, 产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量130kDa细胞定位细胞核 细胞浆 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human NOS-2:1051-1144/1144 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).Function:Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.Subunit:Homodimer. Binds SLC9A3R1.Tissue Specificity:Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.Similarity:Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.SWISS:P35228Gene ID:4843Database links:Entrez Gene: 4843 HumanEntrez Gene: 18126 MouseEntrez Gene: 24599 RatOmim: 163730 HumanSwissProt: P35228 HumanSwissProt: P29477 MouseSwissProt: Q06518 RatUnigene: 709191 HumanUnigene: 2893 MouseUnigene: 10400 RatImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.合成与降解（Synthesis and Degradation）催化生物体内一氧化氮（NO）生成的酶。分神经型一氧化氮合成的酶（nNOS or NOS-1）、诱导型一氧化氮合成的酶（iNOS or NOS-2）、内皮型一氧化氮合成的酶（eNOS or NOS-3）。产品图片Sample:U251 Cell Lysate at 40 ugPrimary: Anti-iNOS(bs-0162R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 130kDObserved band size: 130kDParaformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (inos) Polyclonal Antibody, Unconjugated (bs-0162R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (iNOS) Polyclonal Antibody, Unconjugated (bs-0162R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated(bs-0162R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated(bs-0162R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
bs-0737R，bioss，缺氧诱导因子1α /低氧诱导因子-1抗体/HIF-1 Alpha
基本售价：980.00元
产品编号bs-0737R英文名称HIF-1 Alpha中文名称缺氧诱导因子1α /低氧诱导因子-1抗体别 名ARNT interacting protein; ARNT-interacting protein; Basic helix loop helix PAS protein MOP1; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF 1alpha; HIF-1-alpha; HIF1 A; HIF1 Alpha; HIF1; HIF1-alpha; HIF1A; HIF1A_HUMAN; Hypoxia inducible factor 1 alpha; Hypoxia inducible factor 1 alpha isoform I.3; Hypoxia inducible factor 1 alpha subunit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor; Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor); Hypoxia inducible factor1alpha; Hypoxia-inducible factor 1-alpha; Hypoxia-inducible factor-1a; Member of PAS protein 1; Member of PAS superfamily 1; Member of the PAS Superfamily 1; MOP 1; MOP1; PAS domain-containing protein 8; PASD 8; PASD8. Specific References (22) | bs-0737R has been referenced in 22 publications.[IF=5.595] Liu T et al. MicroRNA-493 targets STMN-1 and promotes hypoxia-induced epithelial cell cycle arrest in G/M and renal fibrosis. (2018) FASEB J. Sep 05 WB ; Mouse. PubMed:30183377 [IF=5.08] Zhang, Huimin, et al. "Vascular Normalization Induced by Sinomenine Hydrochloride Results in Suppressed Mammary Tumor Growth and Metastasis." Scientific Reports 5 (2015). IHC-P ; Mouse. PubMed:25749075 [IF=4.91] Fan, Shengjun, et al. "Opposite angiogenic outcome of curcumin against ischemia and Lewis lung cancer models:in silico, in vitro and in vivo studies." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2014). WB ; Mouse. PubMed:24970744 [IF=4.556] Yang X et al. Synthesis and bioevaluation of novel 18FDG-conjugated 2-nitroimidazole derivatives for tumor-hypoxia imaging. Mol Pharm. 2019 May 6;16(5):2118-2128. IHC&IF ; Mouse&Human. PubMed:30964298 [IF=4.539] Talwar H et al. MEK2 Negatively Regulates Lipopolysaccharide-Mediated IL-1β Production through HIF-1α Expression. J Immunol. 2019 Mar 15;202(6):1815-1825. WB&IP ; Mouse. PubMed:30710049 [IF=4.44] Madka, Venkateshwar, et al. "Targeting mTOR and p53 signaling inhibits muscle invasive bladder cancer in vivo." Cancer Prevention Research 9.1 (2016): 53-62. IHC-P ; Mouse. PubMed:26577454 [IF=4.19] Talwar, Harvinder, et al. "MKP-1 negatively regulates LPS-mediated IL-1β production through p38 activation and HIF-1α expression." Cellular Signalling (2017). Mouse. PubMed:28238855 [IF=3.743] Wang D et al. Effects of hypoxia and ASIC3 on nucleus pulposus cells: From cell behavior to molecularmechanism. Biomed Pharmacother. 2019 Jun 12;117:109061. WB ; Rabbit. PubMed:31202172 [IF=3.53] Woolf, Eric C., et al. "The Ketogenic Diet Alters the Hypoxic Response and Affects Expression of Proteins Associated with Angiogenesis, Invasive Potential and Vascular Permeability in a Mouse Glioma Model." PLOS ONE10.6 (2015): e0130357. WB ; Mouse. PubMed:26083629 [IF=3.288] Chai D et al. β2-microglobulin has a different regulatory molecular mechanism between ER+ and ER- breast cancer with HER2.BMC Cancer. 2019 Mar 12;19(1):223. IHC-P ; Human. PubMed:30866857 [IF=2.989] Ju X et al. Catalpol Promotes the Survival and VEGF Secretion of Bone Marrow-Derived Stem Cells and Their Role in Myocardial Repair After Myocardial Infarction in Rats.Cardiovasc Toxicol. 2018 May 11. WB ; Rat. PubMed:29752623 [IF=2.91] Shou, Zhu, et al. "Expression and prognosis of FOXO3a and HIF-1?? in nasopharyngeal carcinoma."Journal of cancer research and clinical oncology 138.4 (2012): 585-593.. WB ; Human. PubMed:22209974 [IF=2.784] Yang D et al. Normobaric oxygen inhibits AQP4 and NHE1 expression in experimental focal ischemic stroke. (2018) Int. J. Mol. Med. WB ; Rat . PubMed:30592266 [IF=2.766] Dao DT et al. A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor.(2018) PLoS One. WB ; Mouse. PubMed:30566445 [IF=2.566] Yang Z et al. Tenascin-C is involved in promotion of cancer stemness via the Akt/HIF1ɑ axis in esophageal squamous cell carcinoma.Exp Mol Pathol. 2019 Mar 20. WB ; Human. PubMed:30904401 [IF=2.49] Yang, Ya, et al. "Expression of RAP1B is associated with poor prognosis and promotes an aggressive phenotype in gastric cancer." Oncology reports 34.5 (2015): 2385-2394. IHC-P ; Human. PubMed:26329876 [IF=1.64] Guo, Wei, et al. "Transplantation of endothelial progenitor cells in treating rats with IgA nephropathy." BMC Nephrology 15.1 (2014): 110. WB ; Rat. PubMed:25012471 [IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082. WB ; Rat. PubMed:27665780 [IF=1.41] Song et al. Effects of HSYA on the proliferation and apoptosis of MSCs exposed to hypoxic and serum deprivation conditions. (2018) Exp.Ther.Med. 15:5251-5260 WB ; Rat. PubMed:29904409 [IF=.5] Talwar, Harvinder, et al. "The dataset describes: HIF-1 α expression and LPS mediated cytokine production in MKP-1 deficient bone marrow derived murine macrophages." Data in Brief (2017). WB ; Mouse. PubMed:28765831 [IF=0] Talwar et al. The dataset describes: HIF-1 α expression and LPS mediated cytokine production in MKP-1 deficient bone marrow derived murine macrophages. (2017) Data.Brie. 14:56-61 WB ; Mouse. PubMed:28765831 [IF=3.414] Li ZH et al. You-Gui-Yin improved the reproductive dysfunction of male rats with chronic kidney disease via regulating the HIF1α-STAT5 pathway. J Ethnopharmacol. 2020 Jan 10;246:112240. WB ; Rat. PubMed:31526861 研究领域肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, 产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量92kDa细胞定位细胞核 细胞浆 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from middle of human HIF-1 Alpha:341-450/826 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator(ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.Function:Functions as a master transcriptional regulator of theadaptive response to hypoxia. Under hypoxic conditions activatesthe transcription of over 40 genes, including, erythropoietin,glucose transporters, glycolytic enzymes, vascular endothelialgrowth factor, and other genes whose protein products increaseoxygen delivery or facilitate metabolic adaptation to hypoxia.Plays an essential role in embryonic vascularization, tumorangiogenesis and pathophysiology of ischemic disease. Binds to coreDNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment oftranscriptional coactivators such as CREBPB and EP300. Activity isenhanced by interaction with both, NCOA1 or NCOA2. Interaction withredox regulatory protein APEX seems to activate CTAD andpotentiates activation by NCOA1 and CREBBP.Subunit:Interacts with COPS5 subunit of COP9 signalosome complex,leading to the regulation of its stability. Interacts with TSGA10(By similarity). Efficient DNA binding requires heterodimerizationof an alpha and a beta/ARNT subunit. Binds to the TAZ-type 1domains of CREBBP and EP300. Interacts with NCOA1, NCOA2, APEX andHSP90. Interacts with VHL which docks HFA1 to the E3 ubiquitinligase complex for subsequent destruction. Interaction, via the ODDdomain, with the beta domain of VHLL, protects HIF1A fromdestruction by competing against the destructive targetinginitiated by VHL.Subcellular Location:Cytoplasm. Nucleus.Tissue Specificity:Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.Post-translational modifications:In normoxia, is hydroxylated on Pro-402 and Pro-564 in theoxygen-dependent degradation domain (ODD) by EGLN1/PHD1 andEGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564.The hydroxylated prolines promote interaction with VHL, initiatingrapid ubiquitination and subsequent proteasomal degradation. Underhypoxia, proline hydroxylation is impaired and ubiquitination isattenuated, resulting in stabilization.In normoxia, is hydroxylated on Asn-803 by HIF1AN, thusabrogating interaction with CREBBP and EP300 and preventingtranscriptional activation.S-nitrosylation of Cys-800 may be responsible for increasedrecruitment of p300 coactivator necessary for transcriptionalactivity of HIF-1 complex.Acetylation of Lys-532 by ARD1 increases interaction with VHLand stimulates subsequent proteasomal degradation.Requires phosphorylation for DNA-binding.Similarity:Contains 1 basic helix-loop-helix (bHLH) domain.Contains 1 PAC (PAS-associated C-terminal) domain.Contains 2 PAS (PER-ARNT-SIM) domains.SWISS:Q16665Gene ID:3091Database links:Entrez Gene: 3091 HumanEntrez Gene: 15251 MouseOmim: 603348 HumanSwissProt: Q16665 HumanSwissProt: Q61221 MouseUnigene: 597216 HumanUnigene: 3879 MouseUnigene: 446610 MouseImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.缺氧诱导因子1α不仅对于机体在缺氧条件下维持正常的生理功能具有特别重要的意义,并在肿瘤的生长以及神经细胞凋亡等病理过程中起重要作用. HIF1 alpha能调节许多下游基因的表达水平.哺乳动物细胞在低氧压力条件下出现HIF。HIF是一种转录因子，对细胞的缺氧起稳定作用。产品图片Sample:Lane 1: Hela (Human) Cell Lysate at 30 ugLane 2: A431 (Human) Cell Lysate at 30 ugLane 3: U251 (Human) Cell Lysate at 30 ugLane 4: HepG2 (Human) Cell Lysate at 30 ugPrimary: Anti-HIF-1 Alpha (bs-0737R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 120 kDObserved band size: 120 kDParaformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (bs-0737R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (bs-0737R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-HIF-1-Alpha Polyclonal Antibody, Unconjugated(bs-0737R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: rat lung tissue(Smoking); 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-HIF-1-Alpha Polyclonal Antibody, Unconjugated(bs-0737R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingHela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, Unconjugated (bs-0737R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Blank control (blue line): Hela (blue).Primary Antibody (green line): Rabbit Anti- HIF-1 Alpha antibody (bs-0737R)Dilution: 1μg /10^6 cells;Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITCDilution: 1μg /test.ProtocolThe cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
bs-0578R，bioss，I型胶原蛋白/胶原蛋白1/1型胶原蛋白/I型胶原 Collagen I
基本售价：880.00元
产品编号bs-0578R英文名称Collagen I中文名称I型胶原蛋白/胶原蛋白1/1型胶原蛋白/I型胶原别 名Collagen type I; Alpha 1 type I collagen; Alpha 2 type I collagen; COL1A1; COL1A2; Collagen I alpha 1 polypeptide; Collagen I alpha 2 polypeptide; Collagen Of Skin Tendon And Bone; Collagen Type 1; Collagen type I alpha 1; Collagen type I alpha 2; OI4; Osteogenesis Imperfecta Type IV; Pro alpha 1(I) collagen; Type I procollagen; CO1A1_HUMAN; Collagen alpha-1(II) chain; Alpha-1 type II collagen; Collagen alpha-1(II) chain; Chondrocalcin; collagen alpha-1(I) chain preproprotein; Collagen Ⅰ. Specific References (31) | bs-0578R has been referenced in 31 publications.[IF=7.7] Yang, Fan, et al. "Strontium enhances osteogenic differentiation of mesenchymal stem cells and in vivo bone formation by activating Wnt/catenin signaling." Stem Cells 29.6 (2011): 981-991. Human, Rat. PubMed:21563277[IF=5.1] Yu, Chengyuan, et al. "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." Oncotarget 8.23 (2017): 37705. WB ; Dog. PubMed:28465478[IF=4.938] Li Y et al. Klotho recovery by genistein via promoter histone acetylation and DNA demethylation mitigates renal fibrosis in mice.J Mol Med (Berl). 2019 Apr;97(4):541-552. WB ; Mouse&Human. PubMed:30806715[IF=4.45] Wang, Chen-Chie, et al. "Expandable Scaffold Improves Integration of Tissue Engineered Cartilage: An In Vivo Study in A Rabbit Model." Tissue Engineering ja (2016). IHC-P ; Rabbit. PubMed:27193498[IF=4.427] Wu M et al. Synergistic effects of sulfur dioxide and polycyclic aromatic hydrocarbons on pulmonary pro-fibrosis via mir-30c-1-3p/transforming growth factor β type II receptor axis.(2018) Chemosphere 219 WB ; Mouse&Human. PubMed:30543962[IF=4.302] Ju B et al. miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepaticstellate cells. J Cell Mol Med. 2019 Apr 3. WB ; Human. PubMed:30945448[IF=3.952] Yang KC et al. Low-adhesive ethylene vinyl alcohol-based packaging to xenogeneic islet encapsulation for type 1 diabetes treatment.Biotechnol Bioeng. 2018 Sep;115(9):2341-2355. IHC-P ; Rat. PubMed:29777589[IF=3.909] Kara A et al. Bioactive fish scale incorporated chitosan biocomposite scaffolds for bone tissue engineering. Int J Biol Macromol. 2019 Feb 20;130:266-279. SEM, fluorescence microscopy&IHC ; Fish(Sparus aurata). PubMed:30797008[IF=3.82] Luo, Yang, et al. "Alendronate Retards the Progression of Lumbar Intervertebral Disc Degeneration in Ovariectomized Rats." Bone (2013). IHC-P ; Rat. PubMed:23500174[IF=3.61] Xu, Zhen E., et al. "Inflammatory stress exacerbates lipid-mediated renal injury in ApoE/CD36/SRA triple knockout mice." American Journal of Physiology-Renal Physiology 301.4 (2011): F713-F722. IHC-P ; Mouse. PubMed:21795641[IF=3.55] Guo, Hong-Feng, et al. "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." Colloids and Surfaces B: Biointerfaces (2012). WB ; Mouse. PubMed:22503631[IF=3.42] Cecen, Berivan, et al. "Biocompatibility and biomechanical characteristics of loofah based scaffolds combined with hydroxyapatite, cellulose, poly-l-lactic acid with chondrocyte-like cells." Materials Science and Engineering: C(2016). IHC ; Human. PubMed:27612733[IF=3.405] Wu Y et al. MFAP5 promotes basal-like breast cancer progression by activating the EMT program.Cell Biosci. 2019 Mar 7;9:24. WB ; Human. PubMed:30899449[IF=3.373] Niu L et al. PLGA/PLCA casting and PLGA/PDPA electrospinning bilayer film for prevention of postoperative adhesion. (2018) J. Biomed. Mater. Res. Part B Appl. Biomater. IHC-P ; Rabbit. PubMed:30548816[IF=2.79] Yang, Ning, et al. "Caffeic acid phenethyl ester up-regulates antioxidant levels in hepatic stellate cell line T6 via an Nrf2-mediated mitogen activated protein kinases pathway." World J Gastroenterol 23.7 (2017): 1203-1214. WB ; Rat. PubMed:28275300[IF=2.16] Zhou, Nan, et al. "Involvement of Imperatorin Derivative‐OW1 in the antihypertensive effect and vascular remodeling of renovascular hypertension rats." Clinical and Experimental Pharmacology and Physiology (2014). IHC-P ; Rat. PubMed:24798462[IF=2.134] Wang et al. The influence of hypoxia during different pregnancy stages on cardiac collagen accumulation in the adult offspring. (2014) Biomed.Res.In. 2014:419805 WB ; Rat. PubMed:25013780[IF=2.07] Chen, Cheng, et al. "Effects of Cytoskeleton Modulation on the Phenotype of Articular Chondrocytes in Monolayer and Pellet Culture." Journal of Biomaterials and Tissue Engineering 5.12 (2015): 942-950. IHC-P ; Rabbit. PubMed:24116978[IF=1.97] Zhang, Qiang-Ye, et al. "Administration of a novel penicillamine-bound membrane: a preventive and therapeutic treatment for abdominal adhesions." BMC surgery 11.1 (2011): 5. Rat. PubMed:21349198[IF=1.93] Gao, Yuanyuan, et al. "TGF‐β1 promotes bovine mammary fibroblast proliferation through the ERK 1/2 signalling pathway." Cell biology international (2016). IHC-P ; Bovine. PubMed:27063575[IF=1.922] Chi et al. Transdermal estrogen gel and oral aspirin combination therapy improves fertility prognosis via the promotion of endometrial receptivity in moderate to severe intrauterine adhesion. (2018) Mol.Med.Rep. 17:6337-6344 IHC ; Human. PubMed:29512784[IF=1.792] Huo YT et al. Methacholine induces extracellular matrix production by human airway smooth muscle cells through -catenin signaling.Respiratory Physiology & Neurobiology, 254, 55–63. IF ; Human. PubMed:10.1016/j.resp.2018.04.010[IF=1.68] Ren, Xiang, et al. "Engineering zonal cartilage through bioprinting collagen type II hydrogel constructs with biomimetic chondrocyte density gradient."BMC Musculoskeletal Disorders 17.1 (2016): 1. IHC-P ; Rabbit. PubMed:27439428[IF=1.564] Liu P et al. Matrine Suppresses Pancreatic Fibrosis by Regulating TGF-β/Smad Signaling in Rats. (2019) Yonsei Med. J. 60(1) IHC-P ; Rat . PubMed:30554494[IF=1.28] Tian, Fa?Ming, et al. "Orally administered simvastatin partially preserves lumbar vertebral bone mass but not integrity of intervertebral discs in ovariectomized rats." Experimental and Therapeutic Medicine. IHC-P ; Rat. PubMed:28450913[IF=.859] Jiang B et al. Expression of specificity protein 1 and collagen I in primary pterygial tissues. Arq Bras Oftalmol. 2019 Jun 3. WB ; Human. PubMed:31166424[IF=.68] ?akir-?zkan, Nilüfer, et al. "Therapeutic Effects of Sildenafil on Experimental Mandibular Fractures." Journal of Craniofacial Surgery 27.3 (2016): 615-620. IHC-P ; Rat. PubMed:27046469[IF=0] Yang et al. Isolation and biological characterization of tendon-derived stem cells from fetal bovine. (2016) In.Vitro.Cell.Dev.Biol.Ani. 52:846-56 IF(ICC) ; Rat. PubMed:27130678[IF=0] Hendrijantini N et al. Human Umbilical Cord Mesenchymal Stem-Cell Therapy to Increase the Density of Osteoporotic Mandibular Bone. Eur J Dent. 2019 Feb;13(1):58-63. IHC ; Rat. PubMed:31170758[IF=] Luo P et al. IL-37b alleviates inflammation in the temporomandibular joint cartilage via IL-1R8 pathway. Cell Prolif. 2019 Sep 27:e12692. IHC-P ; Rat. PubMed:31560411[IF=3.024] Chen Y et al. An improved cellular enucleation method with extracellular matrix and colchicine facilitates the study of nucleocytoplasmic interaction. Eur J Cell Biol. 2019 Sep 17:151045. ICF ; Human. PubMed:31563281研究领域细胞生物 免疫学 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep, 产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量130kDa细胞定位细胞外基质 分泌型蛋白 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human Collagen I C-terminal propeptide:1321-1400/1464 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed产品介绍This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008].Function:Type I collagen is a member of group I collagen (fibrillar forming collagen).Subunit:Trimers of one alpha 2(I) and two alpha 1(I) chains. Interacts with MRC2. Interacts with TRAM2.Subcellular Location:Secreted, extracellular space, extracellular matrix.Tissue Specificity:Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.Post-translational modifications:Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.DISEASE:Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.Defects in COL1A1 are a cause of osteogenesis imperfecta type 2 (OI2) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.Similarity:Belongs to the fibrillar collagen family.Contains 1 fibrillar collagen NC1 domain.Contains 1 VWFC domain.SWISS:P02452Gene ID:1277Database links:· Entrez Gene: 1277 Human· Entrez Gene: 12842 Mouse· Entrez Gene: 100008952 Rabbit· Entrez Gene: 29393 Rat· Omim: 120150 Human· SwissProt: P02453 Cow· SwissProt: O46392 Dog· SwissProt: P02452 Human· SwissProt: P11087 Mouse· SwissProt: P02454 Rat· Unigene: 172928 Human· Unigene: 277735 Mouse· Unigene: 107239 RatImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.I型胶原是软骨基质中的一种结构蛋白 。主要用于Ⅰ型胶原分布及变态反应方面的研究。产品图片Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Collagen I Polyclonal Antibody, Unconjugated(bs-0578R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Collagen I Polyclonal Antibody, Unconjugated(bs-0578R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: rabbit meniscus tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Collagen I Polyclonal Antibody, Unconjugated(bs-0578R) 1:300, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
bs-10589R，bioss，Ⅱ型胶原蛋白抗体 Collagen II
基本售价：980.00元
产品编号bs-10589R英文名称Collagen II中文名称Ⅱ型胶原蛋白抗体别 名Collagen II alpha 1; COL2A1; COL2A1 protein; collagen, type II, alpha 1; collagen alpha-1(II); type II collagen; alpha-1 type II collagen; alpha1 type II collagen; Col2a1; AOM; Cartilage collagen; Chondrocalcin; COL11A3; Collagen alpha 1(II) chain precursor; Collagen II alpha 1 polypeptide; Collagen type II alpha 1 (primary osteoarthritis spondyloepiphyseal dysplasia congenital); MGC131516; SEDC; CO2A1_HUMAN. Specific References (7) | bs-10589R has been referenced in 7 publications.[IF=4.263] Wu X et al.The role of Ca 2+ in acid-sensing ion channel 1a-mediated chondrocyte pyroptosis in rat adjuvant arthritis.(2018) Lab Invest. WB ; Rat . PubMed:30487596 [IF=3.76] Shi, Yang, et al. "Hypoxia combined with spheroid culture improves cartilage specific function in chondrocytes." Integrative Biology 7.3 (2015): 289-297. Rat. PubMed:25614382 [IF=2.784] Ma C et al.Isolation and biological characteristic evaluation of a novel type of cartilage stem/progenitor cell derived from Small?tailed Han sheep embryos.Int J Mol Med. 2018 Jul;42(1):525-533. ICF ; Sheep. PubMed:29693133 [IF=2.348] Ren et al. Treatment of rabbit intervertebral disc degeneration with co-transfection by adeno-associated virus-mediated SOX9 and osteogenic protein-1 double genes in vivo. (2013) Int.J.Mol.Me. 32:1063-8 WB ; Rabbit. PubMed:24045878 [IF=0] Xu et al. Treatment with SiMiaoFang, an anti-arthritis chinese herbal formula, inhibits cartilage matrix degradation in osteoarthritis rat model. (2013) Rejuvenation.Re. 16:364-76 IHC-P ; Rat. PubMed:23799821 [IF=0] Yang et al. Isolation and biological characterization of tendon-derived stem cells from fetal bovine. (2016) In.Vitro.Cell.Dev.Biol.Ani. 52:846-56 IF(ICC) ; Rat. PubMed:27130678 [IF=] Luo P et al. IL-37b alleviates inflammation in the temporomandibular joint cartilage via IL-1R8 pathway. Cell Prolif. 2019 Sep 27:e12692. IHC-P ; Rat. PubMed:31560411 研究领域肿瘤 细胞生物 免疫学 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, 产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量117kDa细胞定位细胞外基质 分泌型蛋白 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human Collagen II:231-330/1487 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some antibodies with denatured collagen or formalin fixed, paraffin embedded tissues. Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.Function:Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.Subunit:Homotrimers of alpha 1(II) chains.Subcellular Location:Secreted, extracellular space, extracellular matrix.Tissue Specificity:Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.Post-translational modifications:Probably 3-hydroxylated on prolines by LEPREL1 (By similarity). Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2.DISEASE:Defects in COL2A1 are the cause of spondyloepiphyseal dysplasia congenital type (SEDC) [MIM:183900]. This disorder is characterized by disproportionate short stature and pleiotropic involvement of the skeletal and ocular systems.Defects in COL2A1 are the cause of spondyloepimetaphyseal dysplasia Strudwick type (SEMD-STR) [MIM:184250]. A bone disease characterized by disproportionate short stature from birth, with a very short trunk and shortened limbs, and skeletal abnormalities including lordosis, scoliosis, flattened vertebrae, pectus carinatum, coxa vara, clubfoot, and abnormal epiphyses or metaphyses. A distinctive radiographic feature is irregular sclerotic changes, described as dappled in the metaphyses of the long bones.Defects in COL2A1 are the cause of achondrogenesis type 2 (ACG2) [MIM:200610]; also known as achondrogenesis-hypochondrogenesis type II. ACG2 is a disease characterized by the absence of ossification in the vertebral column, sacrum and pubic bones.Defects in COL2A1 are the cause of Legg-Calve-Perthes disease (LCPD) [MIM:150600]; also known as Legg-Perthes disease or Perthes disease. LCPD is characterized by loss of circulation to the femoral head, resulting in avascular necrosis in a growing child. Clinical pictures of the disease vary, depending on the phase of disease progression through ischemia, revascularization, fracture and collapse, and repair and remodeling of the bone.Defects in COL2A1 are the cause of Kniest dysplasia (KD) [MIM:156550]; also known as Kniest syndrome or metatropic dwarfism type II. KD is a moderately severe chondrodysplasia phenotype that results from mutations in the COL2A1 gene. Characteristics of the disorder include a short trunk and extremities, mid-face hypoplasia, cleft palate, myopia, retinal detachment, and hearing loss.Defects in COL2A1 are a cause of primary avascular necrosis of femoral head (ANFH) [MIM:608805]; also known as ischemic necrosis of the femoral head or osteonecrosis of the femoral head. ANFH causes disability that often requires surgical intervention. Most cases are sporadic, but families in which there is an autosomal dominant inheritance of the disease have been identified. It has been estimated that 300,000 to 600,000 people in the United States have ANFH. Approximately 15,000 new cases of this common and disabling disorder are reported annually. The age at the onset is earlier than that for osteoarthritis. The diagnosis is typically made when patients are between the ages of 30 and 60 years. The clinical manifestations, such as pain on exertion, a limping gait, and a discrepancy in leg length, cause considerable disability. Moreover, nearly 10 percent of the 500,000 total-hip arthroplasties performed each year in the United States involve patients with ANFH. As a result, this disease creates a substantial socioeconomic cost as well as a burden for patients and their families.Defects in COL2A1 are the cause of osteoarthritis with mild chondrodysplasia (OACD) [MIM:604864]. Osteoarthritis is a common disease that produces joint pain and stiffness together with radiologic evidence of progressive degeneration of joint cartilage. Some forms of osteoarthritis are secondary to events such as trauma, infections, metabolic disorders, or congenital or heritable conditions that deform the epiphyses or related structures. In most patients, however, there is no readily identifiable cause of osteoarthritis. Inheritance in a Mendelian dominant manner has been demonstrated in some families with primary generalized osteoarthritis. Reports demonstrate coinheritance of primary generalized osteoarthritis with specific alleles of the gene COL2A1, the precursor of the major protein of cartilage.Defects in COL2A1 are the cause of platyspondylic lethal skeletal dysplasia Torrance type (PLSD-T) [MIM:151210]. Platyspondylic lethal skeletal dysplasias (PLSDs) are a heterogeneous group of chondrodysplasias characterized by severe platyspondyly and limb shortening. PLSD-T is characterized by varying platyspondyly, short ribs with anterior cupping, hypoplasia of the lower ilia with broad ischial and pubic bones, and shortening of the tubular bones with splayed and cupped metaphyses. Histology of the growth plate typically shows focal hypercellularity with slightly enlarged chondrocytes in the resting cartilage and relatively well-preserved columnar formation and ossification at the chondro-osseous junction. PLSD-T is generally a perinatally lethal disease, but a few long-term survivors have been reported.Defects in COL2A1 are the cause of multiple epiphyseal dysplasia with myopia and conductive deafness (EDMMD) [MIM:132450]. Multiple epiphyseal dysplasia is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDMMD is an autosomal dominant disorder characterized by epiphyseal dysplasia associated with progressive myopia, retinal thinning, crenated cataracts, conductive deafness.Defects in COL2A1 are the cause of spondyloperipheral dysplasia (SPD) [MIM:271700]. SPD patients manifest short stature, midface hypoplasia, sensorineural hearing loss, spondyloepiphyseal dysplasia, platyspondyly and brachydactyly.Similarity:Belongs to the fibrillar collagen family.Contains 1 fibrillar collagen NC1 domain.Contains 1 VWFC domain.SWISS:P02458Gene ID:1280Database links:Entrez Gene: 1280 HumanEntrez Gene: 12824 MouseEntrez Gene: 25412 RatOmim: 120140 HumanSwissProt: P02458 HumanSwissProt: P28481 MouseSwissProt: P05539 RatUnigene: 408182 HumanUnigene: 2423 MouseUnigene: 10124 RatImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品图片Sample:293T(Human) Cell Lysate at 30 ugA549 (Human) Cell Lysate at 30 ugLOVO (Human) Cell Lysate at 40 ugMCF-7 (Human) Cell Lysate at 30 ugHepG2 (Human) Cell Lysate at 30 ugPrimary: Anti-Collagen II (bs-10589R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 117 kDObserved band size: 117 kDSample:MG63 (Human) Cell Lysate at 30 ugPrimary: Anti-Collagen II (bs-10589R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 117 kDObserved band size: 117 kDParaformaldehyde-fixed, paraffin embedded (mouse ear); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Collagen II) Polyclonal Antibody, Unconjugated (bs-10589R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated(bs-10589R) 1:100, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
bs-0127R，bioss，Bax抗体 Bax
基本售价：980.00元
产品编号bs-0127R英文名称Bax中文名称Bax抗体别 名apoptosis regulator BAX; Apoptosis regulator BAX cytoplasmic isoform beta; Apoptosis regulator BAX membrane isoform alpha; Bax isoform psi; BAX protein cytoplasmic isoform delta; Bax protein cytoplasmic isoform delta. antibody Bax protein cytoplasmic isoform gamma; Bax zeta; Bax-protein; Bcl-2-like protein 4; BCL2 associated X protein; BCL2L4; BAX_HUMAN; Bcl2-L-4. Specific References (68) | bs-0127R has been referenced in 68 publications.[IF=7.519] Goreczny,et al.Hic-5 regulates fibrillar adhesion formation to control tumor extracellular matrix remodeling through interaction with tensin1.(2018) Oncogene. 37:1699-1713. IF(ICC) ; Human. PubMed:29348458 [IF=6.375] Zhou,et al.CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.(2018) Cancer Letters. 418:196-203. IHC-P + WB ; Mouse. PubMed:29317253 [IF=6.217] Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93. WB ; Mouse. PubMed:30791957 [IF=5.62] Qiu, Shi, et al. "A small peptide derived from p53 linker region can resume the apoptotic activity of p53 by sequestering iASPP with p53." Cancer Letters 356.2 (2015): 910-917. IP ; Human. PubMed:25444901 [IF=5.29] Huang, Chuen-Lin, et al. "Paraquat Induces Cell Death Through Impairing Mitochondrial Membrane Permeability." Molecular Neurobiology (2015): 1-20. Rat. PubMed:25947082 [IF=4.59] Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458 WB ; Human. PubMed:26881030 [IF=4.59] Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458 WB ; Human. PubMed:26881030 [IF=4.47] Park, Min-Jung, et al. "New role of irisin in hepatocytes: The protective effect of hepatic steatosis in vitro." Cellular Signalling (2015). WB ; Mouse. PubMed:25917316 [IF=4.12] Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543 WB ; Human. PubMed:27824118 [IF=3.909] Zhu B et al. The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats.Int J Biol Macromol. 2019 Jun 15;131:1-9. IHF ; Rat. PubMed:30851331 [IF=3.83] Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653 WB ; Human. PubMed:28974930 [IF=3.73] Rani, Neha, et al. "Regulation of Heat Shock Proteins 27 and 70, p-Akt/p-eNOS and MAPKs by Naringin Dampens Myocardial Injury and Dysfunction In Vivo after Ischemia/Reperfusion." PLOS ONE 8.12 (2013): e82577. WB ; Rat. PubMed:24324809 [IF=3.73] Zhu, Zhongling, et al. "Regulation of Cell Transformation by Rb-Controlled Redox Homeostasis." PLOS ONE 9.7 (2014): e102582. WB ; Mouse. PubMed:25019272 [IF=3.7] Lin, Lai-xiang, et al. "Feasibility of β-Sheet Breaker Peptide-H102 Treatment for Alzheimers Disease Based on β-Amyloid Hypothesis." PLoS one 9.11 (2014): e112052. IHC-P ; Mouse. PubMed:25372040 [IF=3.56] He, Xin, et al. "Canonical Wnt signaling pathway contributes to the proliferation and survival in porcine pancreatic stem cells (PSCs)." Cell and tissue research 362.2 (2015): 379-388. WB ; Pig. PubMed:26085341 [IF=3.457] Chai FN et al. Coptisine from Rhizoma coptidis exerts an anti-cancer effect on hepatocellular carcinoma by up-regulating miR-122.Biomed Pharmacother. 2018 Jul;103:1002-1011. WB ; Mouse. PubMed:29710498 [IF=3.412] Chen S et al. Anthocyanins from Lycium ruthenicum Murr. ameliorated D-galactose-induced memory impairment, oxidative stress, and neuroinflammation in adult rats.J Agric Food Chem. 2019 Mar 20;67(11):3140-3149. WB&IHC-P ; Rat. PubMed:30813721 [IF=3.33] Chen, Jie, et al. "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." BMC cancer 12.1 (2012): 464. WB ; Human. PubMed:23050783 [IF=3.33] Tian, Xiao Qiang, et al. "The protective effect of Hyperbaric oxygen and Ginkgo Biloba extract on Aβ25-35-induced oxidative stress and neuronal apoptosis in rats." Behavioural Brain Research (2012). WB ; Rat. PubMed:23266522 [IF=3.265] Zeng Q et al.Long-term arsenite exposure induces testicular toxicity by redox imbalance, G2/M cell arrest and apoptosis in mice.(2018) Toxicology. (18)30396-2. WB ; Mouse. PubMed:30278210 [IF=3.265] Si J et al. Effects of ionizing radiation and HLY78 on the zebrafish embryonic developmental toxicity.(2018) Toxicology. 2018 Oct 12. WB ; zebrafish . PubMed:30321647 [IF=3.23] Daverey, Amita, and Sandeep K. Agrawal. "Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes." Neuroscience 333 (2016): 92-103. WB ; Human. PubMed:27423629 [IF=3.208] Cui H et al. Gamma linolenic acid regulates PHD2 mediated hypoxia and mitochondrial apoptosis in DENinduced hepatocellular carcinoma. Drug Des Devel Ther. 2018 Dec 13;12:4241-4252. WB ; Rat. PubMed:30587920 [IF=3.156] Andharia,et al.Electrophysiological properties of anion exchangers in the luminal membrane of guinea pig pancreatic duct cells.(2018) Pflugers Archiv: European Journal of Physiology. 470:897-907. IHC-P + WB ; Guinea Pig. PubMed:29399744 [IF=3.138] Chen J et al. Effect of artemisinin on proliferation and apoptosis-related protein expression in vivo and in vitro.Saudi J Biol Sci. 2018 Nov;25(7):1488-1493. WB&IHC ; Rat. PubMed:30505200 [IF=3.13] Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017). WB ; Human. PubMed:28887916 [IF=3.105] Yang F et al. Copper induces oxidative stress and apoptosis through mitochondria-mediated pathway in chicken hepatocytes.(2019)Toxicol In Vitro. Feb;54:310-316. WB ; Chicken. PubMed:30389602 [IF=3.06] Li, Guiying, et al. "Gremlin Aggravates Hyperglycemia‐Induced Podocyte Injury by a TGFβ/Smad Dependent Signaling Pathway." Journal of Cellular Biochemistry (2013). WB ; Mouse. PubMed:23553804 [IF=2.86] Liu, Yaping, et al. "Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice." International Journal of Molecular Sciences 17.1 (2016): 96. WB ; Mouse. PubMed:26771609 [IF=2.85] Li et al. A plasma mir-125a-5p as a novel biomarker for Kawasaki disease and induces apoptosis in HUVECs. (2017) PLoS.On. 12:e0175407 WB ; Human. PubMed:28467514 [IF=2.76] Liu, Qiang, et al. "Valproic acid attenuates inflammation of optic nerve and apoptosis of retinal ganglion cells in a rat model of optic neuritis." Biomedicine & Pharmacotherapy(2017). WB ; Rat. PubMed:29198746 [IF=2.75] Ye, Bengui, et al. "Anti-tumor activity and relative mechanism of ethanolic extract of Marsdenia tenacissima (Asclepiadaceae) against human hematologic neoplasm in vitro and in vivo." Journal of Ethnopharmacology (2014). WB ; Human. PubMed:24583069 [IF=2.69] Lin, Caiyu, et al. "Lithocarpus Polystachyus Rehd Leaf Aqueous Extract Inhibits Human Breast Cancer Growth In Vitro and In Vivo." Nutrition and Cancer (2014). WB ; Human. PubMed:24660968 [IF=2.656] Liu et al. Effects of insulin-like growth factor binding protein 3 on apoptosis of cutaneous squamous cell carcinoma cells. (2018) Onco.Targets.Ther. 11:6569-6577 WB ; PubMed:30323629 [IF=2.62] Rahman, Md Mostafizur, et al. "Cytotoxic effects of cadmium and zinc co-exposure in PC12 cells and the underlying mechanism." Chemico-Biological Interactions (2017). WB ; Rat. PubMed:28390674 [IF=2.559] Jia C et al. Enhanced antitumor effect of combination of annexin A1 knockdown and bortezomib treatment in multiple myeloma in vitro and in vivo(2018) Biochem Biophys Res Commun.505(3):720-725 WB ; Mouse. PubMed:30292410 [IF=2.491] ?ahin S et al. Vitamin D protects against hippocampal apoptosis related with seizures induced by kainic acid and pentylenetetrazol in rats.(2018) Epilepsy Res.149 IHC-P ; Rat . PubMed:30584976 [IF=2.38] She, Yue, et al. "Aluminum Trichloride Disorders Bile Acid Secretion and Induces Hepatocyte Apoptosis in Rats." Cell Biochemistry and Biophysics (2015): 1-9. IHC-P ; Rat. PubMed:25578489 [IF=2.38] Abdel-Hamid, Nagwa I., Mona F. El-Azab, and Yasser M. Moustafa. "Macrolide antibiotics differentially influence human HepG2 cytotoxicity and modulate intrinsic/extrinsic apoptotic pathways in rat hepatocellular carcinoma model." Naunyn-Schmiedeberg's Archives of Pharmacology (2017): 1-17. IHC-P ; Rat. PubMed:28070612 [IF=2.27] He, X., et al. "Rapamycin inhibits acrolein‐induced apoptosis by alleviating ROS‐driven mitochondrial dysfunction in male germ cells." Cell Proliferation (2014). WB ; Mouse. PubMed:24483236 [IF=2.17] Huang, Guo-fu, et al. "The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration." Evidence-Based Complementary and Alternative Medicine 2014 (2014). WB ; Rabbit. PubMed:25763091 [IF=1.894] Chi et al. Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model. (2018) Med.Sci.Monit. 24:3946-3957 WB ; Rat. PubMed:29888735 [IF=1.66] Wu et al. Resveratrol induces apoptosis in SGC-7901 gastric cancer cells. (2018) Oncol.Lett. 16:2949-2956 WB ; Human. PubMed:30127883 [IF=1.652] Wang Y et al. Protective effects of astaxanthin on subarachnoid hemorrhage-induced early brain injury: Reduction of cerebral vasospasm and improvement of neuron survival and mitochondrial function.(2019)Acta Histochem. Jan;121(1):56-63. WB ; Rat. PubMed:30392635 [IF=1.63] Aksu, E. H., et al. "Mitigation of paracetamol‐induced reproductive damage by chrysin in male rats via reducing oxidative stress." Andrologia (2016). IHC-P ; Rat. PubMed:26914515 [IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082. WB ; Rat. PubMed:27665780 [IF=1.51] Sun, Y‐L., et al. "Relationship between apoptosis and proliferation in granulosa and theca cells of cystic follicles in sows." Reproduction in domestic animals 47.4 (2012): 601-608. IHC-P ; Pig. PubMed:22023076 [IF=1.39] Chen et al. Suppression effect of recombinant adenovirus vector containing hIL-24 on Hep-2 laryngeal carcinoma cells. (2014) Oncol.Let. 7:771-777 WB ; Human. PubMed:24527085 [IF=1.39] Wang et al. Human cytomegalovirus inhibits apoptosis by regulating the activating transcription factor 5 signaling pathway in human malignant glioma cells. (2014) Oncol.Let. 8:1051-1057 WB ; Human. PubMed:25120656 [IF=1.39] Chen et al. Suppression effect of recombinant adenovirus vector containing hIL-24 on Hep-2 laryngeal carcinoma cells. (2014) Oncol.Let. 7:771-777 WB ; Human. PubMed:24527085 [IF=1.39] Wang et al. Human cytomegalovirus inhibits apoptosis by regulating the activating transcription factor 5 signaling pathway in human malignant glioma cells. (2014) Oncol.Let. 8:1051-1057 WB ; Human. PubMed:25120656 [IF=1.31] Wang, Chunqiang, Wei Ma, and Yuhong Su. "NF-κB Pathway Contributes to Cadmium-Induced Apoptosis of Porcine Granulosa Cells." Biological trace element research (2013): 1-8. WB ; Pig. PubMed:23575899 [IF=1.243] Li W et al. Gallic acid caused cultured mice TM4 Sertoli cells apoptosis and necrosis. (2018) Asian-australas. J. Anim. Sci. Oct 26. WB ; Mouse. PubMed:30381745 [IF=1.14] Wang, J., et al. "Esculetin, a coumarin derivative, exerts in vitro and in vivo antiproliferative activity against hepatocellular carcinoma by initiating a mitochondrial-dependent apoptosis pathway." Brazilian Journal of Medical and Biological Research (2014): 000-000. WB ; Mouse. PubMed:25517918 [IF=1.01] Yang, H., et al. "Lipopolysaccharide-induced dental pulp cell apoptosis and the expression of Bax and Bcl-2 in vitro." Brazilian Journal of Medical and Biological Research 43.11 (2010): 1027-1033. IF(ICC) ; Human. PubMed:20945038 [IF=.88] Li, Jinglong, et al. "Deficits of learning and memory in Hemojuvelin knockout mice." Journal of Veterinary Medical Science (2015). WB ; Mouse. PubMed:26027705 [IF=.44] Sutrisno, Sutrisno, et al. "The effect of genistein on TGF-β signal, dysregulation of apoptosis, cyclooxygenase-2 pathway, and NF-kB pathway in mice peritoneum of endometriosis model." Middle East Fertility Society Journal (2017). IHC-P ; Mouse. PubMed:10.1016/j.mefs.2017.05.002 [IF=.2] Sukohar, Asep, and Mohammad Kanedi. "Mucoxin (Acetogenin) Induce Expression of Pro-Apoptosis Proteins, Bax and P53, in T47D Breast Cancer Cells." Biomedical and Pharmacology Journal 10.2 (2017): 641-649. IF(ICC) ; Human. PubMed:10.13005/bpj/1151 [IF=0] Li et al. Morusin suppresses breast cancer cell growth in vitro and in vivo through C/EBPβ and PPARγ mediated lipoapoptosis. (2015) J.Exp.Clin.Cancer.Res. 34:137 WB ; Human. PubMed:26538209 [IF=0] Zhang et al. Protective Effects of Lycium barbarum Polysaccharides on Testis Spermatogenic Injury Induced by Bisphenol A in Mice. (2014) Evid.Based.Complement.Alternat.Me. 2013:690808 IHC-P ; Mouse. PubMed:24454506 [IF=0] Ma et al. A potential adjuvant chemotherapeutics, 18β-glycyrrhetinic acid, inhibits renal tubular epithelial cells apoptosis via enhancing BMP-7 epigenetically through targeting HDAC2. (2016) Sci.Re. 6:25396 WB ; Human,Mouse. PubMed:27145860 [IF=0] Han et al. Protective effects of tao-Hong-si-wu decoction on memory impairment and hippocampal damage in animal model of vascular dementia. (2015) Evid.Based.Complement.Alternat.Me. 2015:195835 IHC-P ; Rat. PubMed:25821478 [IF=0] Xiang M et al. Biyuanling suppresses the toluene-2, 4-diisocyanate induced allergic rhinitis in guinea pigs. Oncotarget. 2017 Dec 8;9(16):12620-12629. WB&IHC-P ; Guinea pigs. PubMed:29560095 [IF=0] Gao Y et al. Hepatoprotective effects of limb ischemic post-conditioning in hepatic ischemic rat model and liver cancer patients via PI3K/ERK pathways.(2018)Int J Biol Sci. Nov 3;14(14):2037-2050. IHC ; Rat. PubMed:30585267 [IF=0] Li X et al. High level expression of ISG12 (1) promotes cell apoptosis via mitochondrial-dependent pathway and so as to hinder Newcastle disease virus replication.(2019)Vet Microbiol. Jan;228:147-156. WB&IF ; Chicken. PubMed:30593361 [IF=0] Zhang Y et al. Ginsenoside Rg3 prevents cognitive impairment by improving mitochondrial dysfunction in the rat model of Alzheimer's disease. J Agric Food Chem. 2019 Aug 27. WB&IHC-P ; Rat. PubMed:31422666 [IF=] Zou P et al. Mechanisms of Stress-Induced Spermatogenesis Impairment in Male Rats Following Unpredictable Chronic Mild Stress (uCMS). Int. J. Mol. Sci. 2019, 20, 4470. WB ; Rat. PubMed:doi:10.3390/ijms20184470 [IF=0] Chen S et al. Anthocyanins from Lycium ruthenicum Murr. Ameliorated d-Galactose-Induced Memory Impairment, Oxidative Stress, and Neuroinflammation in Adult Rats. J Agric Food Chem. 2019 Mar 20;67(11):3140-3149. WB ; Rat. PubMed:30813721 研究领域细胞生物 信号转导 细胞凋亡 线粒体 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, 产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量21kDa细胞定位细胞浆 细胞膜 线粒体 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human Bax:84-175/192 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008].Function:Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.Subunit:Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2.Subcellular Location:Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.Isoform Beta: Cytoplasm.Isoform Gamma: Cytoplasm.Isoform Delta: Cytoplasm (Potential).Tissue Specificity:Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.Similarity:Belongs to the Bcl-2 family.SWISS:Q07812Gene ID:581Database links:Entrez Gene: 581 HumanEntrez Gene: 12028 MouseEntrez Gene: 24887 RatOmim: 600040 HumanSwissProt: Q07812 HumanSwissProt: Q07813 MouseSwissProt: Q63690 RatUnigene: 624291 HumanUnigene: 19904 MouseUnigene: 10668 RatImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.可识别分子量为21KDa的Bax蛋白，此抗体与Bax有较高特异性，且与Bc1-2及Bc1-X蛋白无交叉反应，Bax、Bc1-2和Bc1-X蛋白是凋亡调节蛋白家庭成员。与Bc1-2和Bc1-X相反，Bax蛋白的过量表达加速细胞凋亡。Bax在组织中广泛表达。Bax与Bc1-2比值的高低可用于判断恶性肿瘤耐药及复发。此抗体用于肿瘤及细胞凋亡等方面的研究。最新的研究表明：Bax可能具有肿瘤抑制作用。产品图片Sample: Brain(Rat)lysate 30ug;Liver(Mouse) lysates, 30ug;Primary: Anti-Bax (bs-0127R) at 1:200;Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000;ECL excitated the fluorescence;Predicted band size : 21kDObserved band size : 21kDSample:Cerebral cortex (Rat) Lysate at 40 ugPrimary: Anti-Bax (bs-0127R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 21 kDObserved band size: 21 kDSample:Hela-UV(Human) Cell Lysate at 30 ugPrimary: Anti-Bax (bs-0127R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 21 kDObserved band size: 23 kDSample:Hela(Human) Cell Lysate at 30 ugPrimary: Anti-Bax (bs-0127R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 21 kDObserved band size: 23 kDParaformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (bs-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (bs-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (bs-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(bs-0127R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(bs-0127R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(bs-0127R) 1:800, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingParaformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (bs-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Overlay histogram showing HL 60 cells stained with bs-0127R (Green line).The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (bs-0127R,1μg/1x10^6 cells) for 30 min at 22℃. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) (bs- 0295G-FITC , Brillant blue line) at 1/200 dilution for 30 min at 22℃. Isotype control antibody was rabbit IgG (polyclonal,bs-0295P,Orange line) (1μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
bsm-33033M，bioss，3-磷酸甘油醛脱氢酶（内参）单克隆抗体GAPDH-Loading Control
基本售价：880.00元
产品编号bsm-33033M英文名称GAPDH-Loading Control中文名称3-磷酸甘油醛脱氢酶（内参）单克隆抗体别 名38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10. 研究领域肿瘤 细胞生物 免疫学 神经生物学 新陈代谢 抗体来源Mouse克隆类型Monoclonal克 隆 号4F8交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Rabbit, Sheep, Hamster, Monkey, 产品应用WB=1:5000-20000 IHC-P=1:100-500 ICC=1:100 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量38kDa细胞定位细胞核 细胞浆 细胞膜 性 状Liquid浓 度1mg/ml免 疫 原Recombinded Human GAPDH : 亚 型IgG纯化方法affinity purified by Protein G储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍Loading ControlGlyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.Function:Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.Subunit:Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS.Subcellular Location:Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.Post-translational modifications:S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.ISGylated (Probable).Sulfhydration at Cys-152 increases catalytic activity.Similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.SWISS:P04406Gene ID:2597Database links:Entrez Gene: 374193 ChickenEntrez Gene: 2597 HumanEntrez Gene: 100042025 MouseEntrez Gene: 14433 MouseEntrez Gene: 24383 RatEntrez Gene: 685186 RatEntrez Gene: 317743 ZebrafishOmim: 138400 HumanSwissProt: P00356 ChickenSwissProt: P04406 HumanSwissProt: P16858 MouseSwissProt: P04797 RatSwissProt: Q5XJ10 ZebrafishImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.GAPDH蛋白几乎在所有组织中都高水平表达，广泛用作Western blot蛋白质标准化的内参,是很好的内参抗体。GAPDH 作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的。在实验中，可能存在总蛋白浓度测定不准确；或者蛋白质样品在电泳前上样时产生的样品间的操作误差；这些误差需要通过测定每个样品中实际转到膜上的GAPDH的含量来进行校正，所以一般的western实验都需要进行内参设置。具体校正的方法就是将每个样品测得的目的蛋白含量与本样品的GAPDH含量相除，得到每个样品目的蛋白的相对含量。然后才进行样品与样品之间的比较。甘油醛-3-磷酸脱氢酶（Glyceraldehyde 3 phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）过程中的关键酶。除了在胞质中作为糖酵解的酶以外，有证据表明哺乳动物细胞中的GAPDH参与了多种胞内生化过程，包括膜融合（membrane fusion）、微管成束（microtubule bundling）、磷酸转移酶（phosphotransferase）激活、核内RNA出核、DNA复制与DNA修复。一些生理因素，诸如低氧（hypoxia）和尿糖（diabetes），可以增加GAPDH在特定细胞中的表达。GAPDH存在于几乎所有的组织中，以高水平持续表达。GAPDH（甘油醛-3-磷酸脱氢酶）是参与糖酵解的一种关键酶，由4个30－40kDa的亚基组成.产品图片Sample:Lane1: Skin (Mouse) Lysate at 40 ugLane2: Testis (Mouse) Lysate at 40 ugLane3: Adrenal gland (Mouse) Lysate at 40 ugLane4: Lung (Rat) Lysate at 30 ugPrimary: Anti-GAPDH (bsm-33033M) at 1/1 000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 38 kDSample:A549 Cell Lysate at 25 ug293T Cell Lysate at 40 ugPrimary: Anti-GAPDH(bsm-33033M)at 1/5000 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 38kDObserved band size: 38kDSample:H9C2 Cell (Rat) Lysate at 40 ugU87MG Cell (Human) Lysate at 40 ugHela Cell (Human) Lysate at 40 ugPrimary: Anti- GAPDH (bsm-33033M) at 1/2 000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 35 kDSample:Lane 1: Cerebrum (Rat) Lysate at 40 ugLane 2: Cerebrum (Rat) Lysate at 40 ugLane 3: Cerebrum (Rat) Lysate at 40 ugLane 4: Cerebrum (Rat) Lysate at 40 ugPrimary:Lane 1: Anti-GAPDH (bsm-33033M) at 1/2000 dilutionLane 2: Anti-GAPDH (bsm-33033M) at 1/5000 dilutionLane 3: Anti-GAPDH (bsm-33033M) at 1/10000 dilutionLane 4: Anti-GAPDH (bsm-33033M) at 1/20000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 36 kDSample:Lane 1: Hela (Human) Lysate at 40 ugLane 2: Hela (Human) Lysate at 40 ugLane 3: Hela (Human) Lysate at 40 ugLane 4: Hela (Human) Lysate at 40 ugPrimary:Lane 1: Anti-GAPDH (bsm-33033M) at 1/2000 dilutionLane 2: Anti-GAPDH (bsm-33033M) at 1/5000 dilutionLane 3: Anti-GAPDH (bsm-33033M) at 1/10000 dilutionLane 4: Anti-GAPDH (bsm-33033M) at 1/20000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 36 kDSample:Lane 1: Hela (Human) Cell Lysate at 30 ugLane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ugLane 3: Cerebrum (Mouse) Lysate at 40 ugLane 4: Cerebrum (Rat) Lysate at 40 ugLane 5: Testis (Mouse) Lysate at 40 ugLane 6: Testis (Rat) Lysate at 40 ugLane 7: Kidney (Mouse) Lysate at 40 ugLane 8: HUVEC (Human) Cell Lysate at 30 ugLane 9: A549 (Human) Cell Lysate at 30 ugLane 10: MCF-7 (Human) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bsm-33033M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 36 kDSample:A431(Human) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ugJurkat(Human) Cell Lysate at 30 ugLOVO(Human) Cell Lysate at 30 ugPrimary: Anti- GAPDH (bsm-33033M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 35 kDSample:Hyperpituitarism (Mouse) Lysate at 40 ugPrimary: Anti- GAPDH (bsm-33033M) at 1/5000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 34 kDParaformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (ascites of bsm-33033M-4E8) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (ascites of bsm-33033M-4E8) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (ascites of bsm-33033M-4E8) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAPDH-Loading Control) monoclonal Antibody, Unconjugated (bsm-33033M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
bs-2188R，Rbioss，3-磷酸甘油醛脱氢酶（内参）抗体GAPDH (Loading Control)
基本售价：880.00元
产品编号bs-2188R英文名称GAPDH (Loading Control)中文名称3-磷酸甘油醛脱氢酶（内参）抗体别 名38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase; Glyceraldehyde 3 phosphate dehydrogenase liver; Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10. Specific References (57) | bs-2188R has been referenced in 57 publications.[IF=0] Zhang et al. Increased stathmin expression strengthens fear conditioning in epileptic rats. (2015) Biomed.Rep. 3:28-32 WB ; Rat. PubMed:25469242 [IF=7.901] Lin S et al. Strategy for hypertrophic scar therapy: Improved delivery of triamcinolone acetonide using mechanically robust tip-concentrated dissolving microneedle array. J Control Release. 2019 May 27;306:69-82. WB ; Rabbit. PubMed:31145948 [IF=7.901] Zhang N et al. Polypeptide-engineered DNA tetrahedrons for targeting treatment of colorectal cancer via apoptosis and autophagy. J Control Release. 2019 Jul 10;309:48-58. WB ; Human. PubMed:31301339 [IF=6.306] Zhou P et al. Histamine-4 receptor antagonist JNJ7777120 inhibits pro-inflammatory microglia and prevents the progression of Parkinson-like pathology and behaviour in a rat model.(2018)Brain Behav. Immun. Nov 05. WB ; Rat. PubMed:30408497 [IF=6.217] Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93. WB ; Mouse. PubMed:30791957 [IF=5.79] Yang et al. Rab5-mediated VE-cadherin internalization regulates the barrier function of the lung microvascular endothelium. (2015) Cell.Mol.Life.Sci. 72:4849-66 WB ; Human. PubMed:26112597 [IF=5.17] Zhang et al. Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence. (2018) Oncotarget. 9:2175-2192 WB ; Rat. PubMed:29416763 [IF=5.005] Li Y et al. Exosomes derived from Toxoplasma gondii stimulate an inflammatory response through JNK signaling pathway. Nanomedicine (Lond). 2018 May;13(10):1157-1168. WB ; Human. PubMed:29542367 [IF=4.46] Yan, Lijie, et al. "Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy." Journal of cardiovascular pharmacology 64.4 (2014): 310-317. WB ; Human. PubMed:24887685 [IF=4.38] Sun, Pengchao, et al. "SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer." International Journal of Nanomedicine 12 (2017): 2657. WB ; Human. PubMed:28435250 [IF=4.258] Steenblock et al. The Cdc42 guanine nucleotide exchange factor FGD6 coordinates cell polarity and endosomal membrane recycling in osteoclasts. (2014) J.Biol.Che. 289:18347-59 WB ; Mouse. PubMed:24821726 [IF=3.974] Liao J et al. Inhibition of Caspase-1-dependent pyroptosis attenuates copper-induced apoptosis in chicken hepatocytes.Ecotoxicol Environ Saf. 2019 Jun 15;174:110-119. WB ; Chicken. PubMed:30822667 [IF=3.895] Shen X et al.Retinoic Acid-Induced Protein 14 (RAI14) Promotes mTOR-Mediated Inflammation Under Inflammatory Stress and Chemical Hypoxia in a U87 Glioblastoma Cell Line.(2018) Cell Mol Neurobiol. WB ; Human. PubMed:30554401 [IF=3.831] Zhang H et al. Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway.Front Pharmacol. 2019 Mar 5;10:175. WB ; Rat. PubMed:30890937 [IF=3.687] Yuan FH et al. microRNA‐30a inhibits the liver cell proliferation and promotes cell apoptosis through the JAK/STAT signaling pathway by targeting SOCS‐1 in rats with sepsis. J Cell Physiol. 2019 Apr 10. WB ; Rat. PubMed:30972748 [IF=3.562] Zhang,et al.Interferon-γ Promotes Neuronal Repair by Transplanted Neural Stem Cells in Ischemic Rats.(2018) Stem Cells and Development. 27:355-366. WB ; Rat. PubMed:29298609 [IF=3.457] Ling L et al. MicroRNA-30e promotes hepatocyte proliferation and inhibits apoptosis in cecal ligation and puncture-induced sepsis through the JAK/STAT signaling pathway by binding to FOSL2.Biomed Pharmacother. 2018 Aug;104:411-419. WB ; Rat. PubMed:29787988 [IF=3.44] Chen B et al. Inhibition of Connexin43 Hemichannels with Gap19 Protect-s Cerebral Ischemia/Reperfusion Injury via the JAK2/STAT3 Pathway in Mice.(2018) Brain Res Bull. WB ; Mouse. PubMed:30593877 [IF=3.412] Yang Q et al. Activation of Nrf2 by phloretin attenuates palmitic acid-induced endothelial cell oxidative stress via AMPK-dependent signaling.(2018) J Agric Food Chem. WB ; Human. PubMed:30525573 [IF=3.205] Liu et al. Surgical incision induces learning impairment in mice partially through inhibition of the brain-derived neurotrophic factor signaling pathway in the hippocampus and amygdala. (2018) Mol.Pain. 14:1744806918805902 WB ; Mouse. PubMed:30232930 [IF=3.144] Liu,et al.Cytoprotective effect and purification of novel antioxidant peptides from hazelnut (C. heterophylla Fisch) protein hydrolysates.(2018) Journal of Functional Foods. 42:203-215. WB ; Human. PubMed:10.1016/j.jff.2017.12.003 [IF=3.04] Ma W et al. A vanillin derivative suppresses the growth of HT29 cells through the Wnt/β-catenin signaling pathway. Eur J Pharmacol. 2019 Apr 15;849:43-49. WB ; Human. PubMed:30707959 [IF=2.78] Chai et al. Hypoxia induces pulmonary arterial fibroblast proliferation, migration, differentiation and vascular remodeling via the PI3K/Akt/p70S6K signaling pathway. (2018) Int.J.Mol.Med. 41:2461-2472 IF ; Rat. PubMed:29436587 [IF=2.669] Qiu,et al.Recombinant human maspin inhibits high glucose-induced oxidative stress and angiogenesis of human retinal microvascular endothelial cells via PI3K/AKT pathway.(2018) Molecular and Cellular Biochemistry. :. WB ; Human. PubMed:29363056 [IF=2.634] Zhang X et al. BMP9 Promotes the Extracellular Matrix of Nucleus Pulposus Cells Via Inhibition of the NotchSignaling Pathway. DNA Cell Biol. 2019 Feb 13. WB ; Human. PubMed:30758228 [IF=2.559] Qian L et al. Upregulated circular RNA circ_0074027 promotes glioblastoma cell growth and invasion by regulating miR-518a-5p/IL17RD signaling pathway. Biochem Biophys Res Commun. 2019 Mar 19;510(4):515-519. WB ; Human. PubMed:30738578 [IF=2.498] Huang H et al. Molecular characterization, expression and cellular localization of CYP17 gene during geese (Anser cygnoides) follicular development. Gene. 2018 Jun 5;658:184-190. WB ; Geese. PubMed:29544766 [IF=2.49] Wang, Hong, et al. "Osthole shows the potential to overcome P-glycoprotein?mediated multidrug resistance in human myelogenous leukemia K562/ADM cells by inhibiting the PI3K/Akt signaling pathway." Oncology reports 35.6 (2016): 3659-3668. WB ; Human. PubMed:27109742 [IF=2.431] Wang L et al. Serious Selenium Deficiency in the Serum of Patients with Kashin–Beck Disease and the Effect of Nano-Selenium on Their Chondrocytes. Biol Trace Elem Res. 2019 Jun 8. WB ; Human. PubMed:31175635 [IF=2.387] Jiang L et al. Astrocytes induce proliferation of oligodendrocyte progenitor cells via connexin 47-mediated activation of Chi3l1 expression. Eur Rev Med Pharmacol Sci. 2019 Apr;23(7):3012-3020. WB ; Rat. PubMed:31002152 [IF=2.314] Ma H et al. Interferon-γ facilitated adjuvant-induced arthritis at early stage. Scand J Immunol. 2019 Feb 9:e12757. WB ; Mouse. PubMed:30739356 [IF=2.3] Zhang, Xiangliang, et al. "MicroRNA-216a enhances the radiosensitivity of pancreatic cancer cells by inhibiting beclin-1-mediated autophagy." Oncology Reports. WB ; PubMed:26134156 [IF=2.14] Song, Yong, et al. "Increased expression of the pluripotency markers sex-determining region Y-box 2 and Nanog homeobox in ovarian endometriosis." Reproductive Biology and Endocrinology 12.1 (2014): 42. WB ; Human. PubMed:24884521 [IF=2.11] Yan, Lijie, et al. "Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy." Journal of cardiovascular pharmacology 64.4 (2014): 310-317. WB ; Human. PubMed:24887685 [IF=2.11] Li et al. All-trans retinoic acid regulates the expression of the extracellular matrix protein fibulin-1 in the guinea pig sclera and human scleral fibroblasts. (2010) Mol.Vi. 16:689-97 WB ; Human. PubMed:20405022 [IF=2.09] Tan, Shifan, et al. "miR-409-3p sensitizes colon cancer cells to oxaliplatin by inhibiting Beclin-1-mediated autophagy." International Journal of Molecular Medicine. WB ; PubMed:26935807 [IF=1.922] Cao et al. Dickkopf?3 upregulation mediates the cardioprotective effects of curcumin on chronic heart failure. (2018) Mol.Med.Rep. 17:7249-7257 WB ; rabbit. PubMed:29568962 [IF=1.92] Huang et al. Intracellular iodinated compounds affect sodium iodide symporter expression through TSH-mediated signaling pathways. (2011) Mol.Med.Rep. 4:77-80 WB ; Porcine. PubMed:21461567 [IF=1.851] Dai X et al. Silencing of MALAT1 inhibits migration and invasion by sponging miR?1?3p in prostate cancer cells. Mol Med Rep. 2019 Aug 22. WB ; Human. PubMed:31485645 [IF=1.632] Wang R et al. Molecular cloning and functional characterization of porcine 2',5'-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory syndrome virus. Vet Immunol Immunopathol. 2019 Mar;209:22-30. WB ; Porcine. PubMed:30885302 [IF=1.563] Wang,et al.Screening of genes involved in epithelial-mesenchymal transition and differential expression of complement-related genes induced by PAX2 in renal tubules.() Nephrology. :. WB ; Rat. PubMed:29280536 [IF=1.55] Jia, Lianqun, et al. "Effects of Tanshinone IIA on the modulation of miR?33a and the SREBP?2/Pcsk9 signaling pathway in hyperlipidemic rats." Molecular Medicine Reports (2016). WB ; Rat. PubMed:27082100 [IF=1.514] Wang Y et al. Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress. Iran J Basic Med Sci. 2018 Dec;21(12):1238-1244. WB ; Rat. PubMed:30627367 [IF=1.48] Hou, Yu Sen, et al. "Lipopolysaccharide pretreatment inhibits LPS-induced human umbilical cord mesenchymal stem cell apoptosis via upregulating the expression of cellular FLICE-inhibitory protein." Molecular Medicine Reports. WB ; Human. PubMed:25955291 [IF=1.358] Ma WF et al. Vanillic acid alleviates palmitic acid‐induced oxidative stress in human umbilical vein endothelial cells via Adenosine Monophosphate‐Activated Protein Kinase signaling pathway. Journal of Food Biochemistry,2019 e12893. WB ; Human. PubMed:doi:10.1111/jfbc.12893 [IF=1.28] YIN, Zhi-hong, et al. "Expression and tissue distribution analysis of Angiotensin II in sheep (Ovis aries) skins associated with white and black coat colors." Journal of Zhejiang University Science B 1 (2016). WB ; Sheep. PubMed:0 [IF=1.189] Xu Q et al. Expression of macrophage migration inhibitory factor in Aspergillus fumigatus keratitis. Int J Ophthalmol. 2019 May 18;12(5):711-716. WB ; Rat&Human. PubMed:31131227 [IF=.99] Shao, Guangcan, et al. "Construction of the plasmid coding for the expression of the EGFP?M?IL?2 (88Arg, 125Ala) fusion protein and the anti?tumor effects exerted by the fusion protein in HeLa-60 cells." Oncology Letters. WB ; Human. PubMed:26137137 [IF=0] Kaushal P et al. Ameliorative role of antioxidant supplementation on sodium-arsenite induced adverse effects on the developing rat cerebellum. Journal of Ayurveda and Integrative Medicine.2019 WB ; Rat. PubMed:doi:10.1016/j.jaim.2018.02.138 [IF=0] Wen Y et al. NADPH Oxidase Hyperactivity Contributes to Cardiac Dysfunction and Apoptosis in Rats with Severe Experimental Pancreatitis through ROS-Mediated MAPK Signaling Pathway. Oxid Med Cell Longev. 2019 May 9;2019:4578175. WB ; Rat. PubMed:31210840 [IF=0] Jiang S et al. Inhibitory Effect of Slit2-N on VEGF165-induced proliferation of vascular endothelia via Slit2-N-Robo4-Akt pathway in choroidal neovascularization. Cell Cycle. 2019 Jun;18(11):1241-1253. WB ; Human. PubMed:31081721 [IF=] Li T et al. Withanolides, extracted from Datura metel L. inhibit keratinocyte proliferation and imiquimod- induced psoriasis-like dermatitis via the STAT3/P38/ERK1/2 pathway. Molecules. 2019 Jul 17;24(14). pii: E2596. WB ; Human. PubMed:31319488 [IF=] Luo P et al. IL-37b alleviates inflammation in the temporomandibular joint cartilage via IL-1R8 pathway. Cell Prolif. 2019 Sep 27:e12692. WB ; Human. PubMed:31560411 [IF=3.266] Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019. WB ; Mouse. PubMed:31497217 [IF=2.926] Wang M et al. Expression and localization of the small proteoglycans decorin and biglycan in articular cartilage of Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency. Glycoconj J. 2019 Sep 2. WB ; Human. PubMed:31478096 [IF=1.851] Duan X et al. Expression of TIGIT/CD155 and correlations with clinical pathological features in human hepatocellular carcinoma. Mol Med Rep. 2019 Oct;20(4):3773-3781. WB ; Human. PubMed:31485637 [IF=.607] Yang F et al. Effect of Bone Morphogenetic Protein 6 (BMP6) on Chicken Granulose Cells Proliferation and Progesterone Synthesis. Brazilian Journal of Poultry Science.2019 Feb. WB ; Chicken. PubMed:doi:10.1590/1806-9061-2018-0835 产品类型内参抗体 研究领域免疫学 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, Rabbit, 产品应用WB=1:2000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量37kDa细胞定位细胞核 细胞浆 细胞膜 性 状Liquid浓 度1mg/ml免 疫 原GAPDH protein of rabbit: 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 38kDa subunits.Function:Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.Subunit:Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS.Subcellular Location:Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.Post-translational modifications:S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.ISGylated (Probable).Sulfhydration at Cys-152 increases catalytic activity.Similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.SWISS:P04406Gene ID:100009074Database links:Entrez Gene: 493876 CatEntrez Gene: 374193 ChickenEntrez Gene: 403755 DogEntrez Gene: 2597 HumanEntrez Gene: 100042025 MouseEntrez Gene: 14433 MouseEntrez Gene: 396823 PigEntrez Gene: 100009074 RabbitEntrez Gene: 24383 RatEntrez Gene: 685186 RatEntrez Gene: 380259 Xenopus laevisEntrez Gene: 448356 Xenopus tropicalisEntrez Gene: 317743 ZebrafishOmim: 138400 HumanSwissProt: CatSwissProt: P00356 ChickenSwissProt: Q28259 DogSwissProt: P70685 Guinea pigSwissProt: P04406 HumanSwissProt: P16858 MouseSwissProt: P00355 PigSwissProt: P46406 RabbitSwissProt: P04797 RatSwissProt: P51469 Xenopus laevisSwissProt: Q5XJ10 ZebrafishUnigene: 544577 HumanUnigene: 592355 HumanUnigene: 598320 HumanUnigene: 304088 MouseUnigene: 309092 MouseUnigene: 317779 MouseUnigene: 343110 MouseUnigene: 392463 MouseUnigene: 392480 MouseUnigene: 414470 MouseUnigene: 458138 MouseUnigene: 458416 MouseUnigene: 475698 MouseUnigene: 129558 RatUnigene: 91450 RatUnigene: 995 Xenopus laevisUnigene: 35640 ZebrafishImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.甘油醛-3-磷酸脱氢酶（Glyceraldehyde 3 phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）过程中的关键酶。除了在胞质中作为糖酵解的酶以外，有证据表明哺乳动物细胞中的GAPDH参与了多种胞内生化过程，包括膜融合（membrane fusion）、微管成束（microtubule bundling）、磷酸转移酶（phosphotransferase）激活、核内RNA出核、DNA复制与DNA修复。一些生理因素，诸如低氧（hypoxia）和尿糖（diabetes），可以增加GAPDH在特定细胞中的表达。GAPDH存在于几乎所有的组织中，以高水平持续表达。 GAPDH（甘油醛-3-磷酸脱氢酶）是参与糖酵解的一种关键酶，由4个30－40kDa的亚基组成。产品图片Sample:U251(Human) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bs-2188R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 37 kDSample:Hela Cell (Human) Lysate at 40 ugMCF-7 Cell (Human) Lysate at 40 ugPrimary: Anti- GAPDH (bs-2188R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 37 kDSample:A549(Human) Cell Lysate at 30 ugU2os(Human) Cell Lysate at 30 ugMolt-4(Human) Cell Lysate at 30 ugA431(Human) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bs-2188R) at 1/2000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 37 kDSample:Heart (Rat) Lysate at 40 ugCerebrum (Rat) Lysate at 40 ugLiver (Mouse) Lysate at 40 ugPrimary: Anti- GAPDH (bs-2188R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 35 kDSample:Liver(Rat) Lysate at 40 ugPrimary: Anti-GAPDH (bs-2188R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 35 kDSample:Kidney (Mouse) Lysate at 40 ugPrimary: Anti-GAPDH (bs-2188R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 35 kDSample:Muscle (Rat) Lysate at 40 ugHeart (Rat) Lysate at 40 ugPrimary: Anti- GAPDH (bs-2188R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 37 kDSample:Lane 1: NIH/3T3(Mouse) Cell Lysate at 30 ugLane 2: Cerebrum (Mouse) Lysate at 40 ugLane 3: Cerebrum (Rat) Lysate at 40 ugLane 4: HUVEC (Human) Cell Lysate at 30 ugLane 5: A549 (Human) Cell Lysate at 30 ugLane 6: MCF-7 (Human) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bs-2188R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 37 kDSample:U251(Human) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ugMCF-7(Human) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bs-2188R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 37 kDParaformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated(bs-2188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated(bs-2188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
BSKV0001，bioss，SARS-CoV-2 (2019-nCoV) Nucleoprotein ELISA Kit
基本售价：4800.00元
产品编号BSKV0001英文名称 SARS-CoV-2 (2019-nCoV) Nucleoprotein ELISA Kit别 名SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein; SARS-CoV-2 N ELISA Kit 线性范围0.78 - 25ng/ml应用范围S/P/CC检测限0.4 ng/ml适用样品基质cell culture supernates and cell culture exctracts保存条件Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)产品介绍Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 为应对 COVID-19疫情的爆发，北京博奥森生物技术有限公司启动了针对新型冠状病毒的科研攻关，成功研发出SARS-CoV-2 (2019-nCoV) Nucleoprotein蛋白检测试剂盒。 该试剂盒根据ELISA双抗体夹心法原理，采用一步法，实现对2019-nCoV抗原蛋白的快速准确检测。试剂盒所采用原料均来自本公司科研团队自主研发，经严苛筛选验证，保证其灵敏度高、特异性好，结果一致且可靠，为疫情的相关疫苗及治疗性药物研究等工作贡献一份力量。产品特色：1 时间短，提高检测效率；2 操作简便，实验误差更??；3 样本需求量小，节约珍贵检测样本；4 灵敏度高及特异性好，结果一致且可靠。产品图片SARS-CoV-2 (2019-nCoV) Nucleoprotein ELISA KitExample of linearity and sensitivity of SARS-CoV-2 (2019-nCoV) Nucleoprotein ELISA Kit
bs-10900R，bioss，3-磷酸甘油醛脱氢酶（内参）抗体 GAPDH (Loading Control)
基本售价：880.00元
产品编号bs-10900R英文名称GAPDH (Loading Control)中文名称3-磷酸甘油醛脱氢酶（内参）抗体别 名38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10. 产品类型内参抗体 研究领域肿瘤 细胞生物 免疫学 信号转导 新陈代谢 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, 产品应用WB=1:5000-20000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量38kDa细胞定位细胞核 细胞浆 细胞膜 性 状Liquid浓 度1mg/ml免 疫 原Recombinant human GAPDH full length protein: 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed产品介绍Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.Function:Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.Subunit:Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS.Subcellular Location:Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.Post-translational modifications:S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.ISGylated (Probable).Sulfhydration at Cys-152 increases catalytic activity.Similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.SWISS:P04406Gene ID:2597Database links:Entrez Gene: 374193 ChickenEntrez Gene: 2597 HumanEntrez Gene: 100042025 MouseEntrez Gene: 14433 MouseEntrez Gene: 24383 RatEntrez Gene: 685186 RatEntrez Gene: 317743 ZebrafishOmim: 138400 HumanSwissProt: P00356 ChickenSwissProt: P04406 HumanSwissProt: P16858 MouseSwissProt: P04797 RatSwissProt: Q5XJ10 ZebrafishImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.GAPDH蛋白几乎在所有组织中都高水平表达，广泛用作Western blot蛋白质标准化的内参,是很好的内参抗体。GAPDH 作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的。在实验中，可能存在总蛋白浓度测定不准确；或者蛋白质样品在电泳前上样时产生的样品间的操作误差；这些误差需要通过测定每个样品中实际转到膜上的GAPDH的含量来进行校正，所以一般的western实验都需要进行内参设置。具体校正的方法就是将每个样品测得的目的蛋白含量与本样品的GAPDH含量相除，得到每个样品目的蛋白的相对含量。然后才进行样品与样品之间的比较。甘油醛-3-磷酸脱氢酶（Glyceraldehyde 3 phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）过程中的关键酶。除了在胞质中作为糖酵解的酶以外，有证据表明哺乳动物细胞中的GAPDH参与了多种胞内生化过程，包括膜融合（membrane fusion）、微管成束（microtubule bundling）、磷酸转移酶（phosphotransferase）激活、核内RNA出核、DNA复制与DNA修复。一些生理因素，诸如低氧（hypoxia）和尿糖（diabetes），可以增加GAPDH在特定细胞中的表达。GAPDH存在于几乎所有的组织中，以高水平持续表达。GAPDH（甘油醛-3-磷酸脱氢酶）是参与糖酵解的一种关键酶，由4个30－40kDa的亚基组成.产品图片Sample:293T(Human) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bs-10900R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 38 kDSample: 293T(human) cell lysate at 30ug;Primary:Lane1: Anti-GAPDH (bs-10900R) at 1/2000 dilutionLane2: Anti-GAPDH (bs-10900R) at 1/10000 dilutionLane3: Anti-GAPDH (bs-10900R) at 1/40000 dilutionLane4: Anti-GAPDH (bs-10900R) at 1/80000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 38kDSample:H9C2(Rat) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bs-10900R) at 1/2000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 38 kDSample:Lane 1: 293T (Human) Lysate at 40 ugLane 2: 293T (Human) Lysate at 40 ugLane 3: 293T (Human) Lysate at 40 ugLane 4: 293T (Human) Lysate at 40 ugPrimary:Lane 1: Anti-GAPDH (bs-10900R) at 1/2000 dilutionLane 2: Anti-GAPDH (bs-10900R) at 1/5000 dilutionLane 3: Anti-GAPDH (bs-10900R) at 1/10000 dilutionLane 4: Anti-GAPDH (bs-10900R) at 1/20000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 36 kDSample:Lane 1: SiHa (Human) Cell Lysate at 30 ugLane 2: NIH/3T3(Mouse) Cell Lysate at 30 ugLane 3: Large intestine (Mouse) Lysate at 40 ugLane 4: Cerebrum (Rat) Lysate at 40 ugLane 5: Cerebrum (Mouse) Lysate at 40 ugLane 6: Testis (Rat) Lysate at 40 ugLane 7: Testis (Mouse) Lysate at 40 ugLane 8: Kidney (Mouse) Lysate at 40 ugLane 9: HUVEC (Human) Cell Lysate at 30 ugLane 10: A549 (Human) Cell Lysate at 30 ugLane 11: MCF-7 (Human) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bs-10900R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 36 kDSample:Heart (Mouse) Lysate at 40 ugCerebrum (Mouse) Lysate at 40 ugLiver (Mouse) Lysate at 40 ugKidney (Mouse) Lysate at 40 ugPrimary: Anti- GAPDH (bs-10900R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 38 kDSample:Lung (Mouse) Lysate at 40 ugSpleen (Mouse) Lysate at 40 ugThymus (Mouse) Lysate at 40 ugPrimary: Anti- GAPDH-Loading Control (bs-10900R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 35 kDSample:A549 Cell (Human) Lysate at 40 ugA431 Cell (Human) Lysate at 40 ugNIH/3T3 Cell (Mouse) Lysate at 40 ugPrimary: Anti-GAPDH (bs-10900R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 36 kDParaformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Contro) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAPDH (Loading Control)) polyclonal Antibody, Unconjugated (bs-10900R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAPDH (Loading Control)) polyclonal Antibody, Unconjugated (bs-10900R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
bs-0081R，bioss，活化半胱胺酸蛋白酶蛋白-3抗体 Caspase-3
基本售价：980.00元
产品编号bs-0081R英文名称Caspase-3中文名称活化半胱胺酸蛋白酶蛋白-3抗体别 名Caspase-3 subunit p17; cleaved Caspase 3; cleaved Caspase-3; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1. Specific References (117) | bs-0081R has been referenced in 117 publications.[IF=9.91] Li, Ting, et al. "Proliferation of parenchymal microglia is the main source of microgliosis after ischaemic stroke." Brain (2013): awt287. Mouse. PubMed:24154617 [IF=8.355] Chen Z et al. Enzyme-powered Janus nanomotors launched from intratumoral depots to address drug delivery barriers. Chemical Engineering Journal,2019 375, 122109. IHC ; Mouse. PubMed:doi:10.1016/j.cej.2019.122109 [IF=7.6] Teng, I., et al. "Phospholipid-functionalized mesoporous silica nanocarriers for selective photodynamic therapy of cancer." Biomaterials (2013). WB ; Mouse. PubMed:23810081 [IF=7.05] He, Nan, et al. "Tumor pH-responsive Release of Drug-conjugated Micelles from Fiber Fragments for Intratumoral Chemotherapy." ACS Applied Materials & Interfaces (2017). other ; PubMed:28876891 [IF=6.375] Zhou,et al.CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.(2018) Cancer Letters. 418:196-203. IHC-P + WB ; Mouse. PubMed:29317253 [IF=6.217] Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93. WB ; Mouse. PubMed:30791957 [IF=6.01] Chen, Zhoujiang, et al. 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The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats.Int J Biol Macromol. 2019 Jun 15;131:1-9. IHF ; Rat. PubMed:30851331 [IF=3.85] Wang, Yandi, et al. "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." Toxicology and Applied Pharmacology (2016). IHC-P ; Sheep. PubMed:27815134 [IF=3.84] Kubatka, Peter, et al. "Fruit peel polyphenols demonstrate substantial anti-tumour effects in the model of breast cancer." European Journal of Nutrition(2015): 1-11. IHC-P ; Rat. PubMed:25930965 [IF=3.83] Yang et al. MicroRNA-145 Increases the Apoptosis of Activated Hepatic Stellate Cells Induced by TRAIL through NF-κB Signaling Pathway. (2018) Front.Pharmacol. 8:980 WB ; Mouse. PubMed:29375381 [IF=3.82] Clark, Andrea J., et al. "WO3/Pt nanoparticles are NADPH oxidase biomimetics that mimic effector cells in vitro and in vivo." Nanotechnology27.6 (2015): 065101. 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Newcastle Disease Virus V Protein Inhibits Cell Apoptosis and Promotes Viral Replication by Targeting CacyBP/SIP. (2018) Front.Cell.Infect.Microbiol. 8:304 WB ; PubMed:30234028 [IF=3.47] Kubatka, Peter, et al. "Oregano demonstrates distinct tumour-suppressive effects in the breast carcinoma model." European Journal of Nutrition (2016): 1-14. IHC-P ; Rat. PubMed:26907089 [IF=3.448] Xu B et al. Excessive mechanical stress induces chondrocyte apoptosis through TRPV4 in an anteriorcruciate ligament-transected rat osteoarthritis model. Life Sci. 2019 Jul 1;228:158-166. WB ; Rat. PubMed:31055086 [IF=3.412] Chen S et al. Anthocyanins from Lycium ruthenicum Murr. ameliorated D-galactose-induced memory impairment, oxidative stress, and neuroinflammation in adult rats.J Agric Food Chem. 2019 Mar 20;67(11):3140-3149. WB&IHC-P ; Rat. PubMed:30813721 [IF=3.4] Gao, Hui, et al. "Hispidulin induces mitochondrial apoptosis in acute myeloid leukemia cells by targeting extracellular matrix metalloproteinase inducer."American Journal of Translational Research 8.2 (2016): 1115-1132 WB ; Human. PubMed:27158398 [IF=3.23] Wang, Yu, et al. "Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes." PloS one 12.4 (2017): e0173469. WB ; Rat. PubMed:28399139 [IF=3.17] Liu, Yang, et al. "Amelioration of Stroke-Induced Neurological Deficiency by Lyophilized Powder of Catapol and Puerarin." International Journal of Biological Sciences 10.4 (2014): 448-456. WB ; Mouse. PubMed:24719562 [IF=3.156] Andharia,et al.Electrophysiological properties of anion exchangers in the luminal membrane of guinea pig pancreatic duct cells.(2018) Pflugers Archiv: European Journal of Physiology. 470:897-907. IHC-P + WB ; Guinea Pig. PubMed:29399744 [IF=3.138] Chen J et al. Effect of artemisinin on proliferation and apoptosis-related protein expression in vivo and in vitro.Saudi J Biol Sci. 2018 Nov;25(7):1488-1493. WB&IHC ; Rat. PubMed:30505200 [IF=3.13] Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017). WB ; Human. PubMed:28887916 [IF=3.105] Yang F et al. Copper induces oxidative stress and apoptosis through mitochondria-mediated pathway in chicken hepatocytes.(2019)Toxicol In Vitro. Feb;54:310-316. WB ; Chicken. PubMed:30389602 [IF=3.06] Li, Guiying, et al. ?Gremlin Aggravates Hyperglycemia‐Induced Podocyte Injury by a TGFβ/Smad Dependent Signaling Pathway.? Journal of CellularBiochemistry (2013). WB ; Mouse. PubMed:23553804 [IF=3.043] Chen Y et al. Conditional deletion of Bmp2 in cranial neural crest cells recapitulates Pierre Robin sequence in mice.(2018)Cell Tissue Res.Nov 9. IHC ; Mouse. PubMed:30413887 [IF=2.959] Song, D. et al. Ivermectin inhibits the growth of glioma cells by inducing cell cycle arrest and apoptosis in vitro and in vivo. (2018) Journal of Cellular Biochemistry. WB ; Rat. PubMed:10.1002/jcb.27420 [IF=2.903] Wang C et al. Newcastle disease virus V protein inhibits apoptosis in DF-1 cells by downregulating TXNL1. (2018) Vet Res. 49(1):102. WB ; chicken embryos. PubMed:30290847 [IF=2.9] Shan, Ming, and Ting-Jun Fan. "Cytotoxicity of carteolol to human corneal epithelial cells by inducing apoptosis via triggering the Bcl-2 family protein-mediated mitochondrial pro-apoptotic pathway." Toxicology in Vitro (2016). ELISA ; Human. PubMed:27216471 [IF=2.86] Liu, Yaping, et al. "Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice." International Journal of Molecular Sciences 17.1 (2016): 96. WB ; Mouse. PubMed:26771609 [IF=2.85] Xu et al. Resveratrol controlled the fate of porcine pancreatic stem cells through the Wnt/β-catenin signaling pathway mediated by Sirt1. (2017) PLoS.On. 12:e0187159 WB ; Pig. PubMed:29073244 [IF=2.791] Wang Q et al. Cdc20 and molecular chaperone CCT2 and CCT5 are required for the Muscovy duck reovirus p10. 8-induced cell cycle arrest and apoptosis. Vet Microbiol. 2019 Jun 28;235:151-163. WB ; Monkey. PubMed:31282373 [IF=2.75] Ye, Bengui, et al. "Anti-tumor activity and relative mechanism of ethanolic extract of Marsdenia tenacissima (Asclepiadaceae) against human hematologic neoplasm in vitro and in vivo." Journal of Ethnopharmacology (2014). WB ; Human. PubMed:24583069 [IF=2.7] Lv, Runxiao, et al. "Neuroprotective effect of allicin in a rat model of acute spinal cord injury." Life Sciences (2015). WB ; Rat. PubMed:26546416 [IF=2.69] Lin, Caiyu, et al. "Lithocarpus Polystachyus Rehd Leaf Aqueous Extract Inhibits Human Breast Cancer Growth In Vitro and In Vivo." Nutrition and Cancer (2014). WB ; Human. PubMed:24660968 [IF=2.656] Liu et al. Effects of insulin-like growth factor binding protein 3 on apoptosis of cutaneous squamous cell carcinoma cells. (2018) Onco.Targets.Ther. 11:6569-6577 WB ; PubMed:30323629 [IF=2.61] Lei, Mingxing, et al. ?Gsdma3 is a new factor needed for TNF-α-mediated apoptosis signal pathway in mouse skin keratinocytes.? Histochemistry and cell biology 138.3 (2012): 385-396. WB, IHC-P ; Mouse. PubMed: 22585037 [IF=2.561] Peng X et al. Overexpression of modified human TRβ1 suppresses the growth of hepatocarcinoma SK-hep1 cells in vitro and in xenograft models.Mol Cell Biochem. 2018 Dec;449(1-2):207-218. IHC-P&WB ; Mouse&Human. PubMed:29679278 [IF=2.535] Chen J et al. Tension induces intervertebral disc degeneration via endoplasmic reticulum stress-mediated autophagy. Biosci Rep. 2019 Jul 8. pii: BSR20190578. WB&IHC ; Rat. PubMed:31285389 [IF=2.53] Yoshinaga, Ayana, et al. "NEU3 inhibitory effect of naringin suppresses cancer cell growth by attenuation of EGFR signaling through GM3 ganglioside accumulation." European Journal of Pharmacology (2016). WB ; Human. PubMed:27105818 [IF=2.52] Yao, Kun, et al. "Knockdown of RLIP76 expression by RNA interference inhibits proliferation, enhances apoptosis, and increases chemosensitivity to daunorubicin in U937 leukemia cells." Tumor Biology (2014): 1-9. WB ; Human. PubMed:24839008 [IF=2.498] Du J et al. MicroRNA-204-5p regulates 3T3-L1 preadipocyte proliferation, apoptosis and differentiation.Gene. 2018 Aug 20;668:1-7. ICF ; Mouse. PubMed:29775748 [IF=2.491] ?ahin S et al. Vitamin D protects against hippocampal apoptosis related with seizures induced by kainic acid and pentylenetetrazol in rats.(2018) Epilepsy Res.149 IHC-P ; Rat . PubMed:30584976 [IF=2.41] Guan, Junhong, et al. "Bone morphogenetic protein-7 (BMP-7) mediates ischemic preconditioning-induced ischemic tolerance via attenuating apoptosis in rat brain." Biochemical and Biophysical Research Communications (2013). WB ; Rat. PubMed:24184479 [IF=2.38] Gao, Hui, et al. "Hispidulin Potentiates the Antitumor Effect of Sunitinib Against Human Renal Cell Carcinoma in Laboratory Models." Cell Biochemistry and Biophysics: 1-8. WB ; Human. PubMed:25260394 [IF=2.38] Gao, Hui, et al. "shRNA-Mediated EMMPRIN Silencing Inhibits Human Leukemic Monocyte Lymphoma U937 Cell Proliferation and Increases Chemosensitivity to Adriamycin." Cell Biochemistry and Biophysics: 1-9. WB ; Human. PubMed:25260396 [IF=2.38] Abdel-Hamid, Nagwa I., Mona F. El-Azab, and Yasser M. Moustafa. "Macrolide antibiotics differentially influence human HepG2 cytotoxicity and modulate intrinsic/extrinsic apoptotic pathways in rat hepatocellular carcinoma model." Naunyn-Schmiedeberg's Archives of Pharmacology (2017): 1-17. IHC-P ; Rat. 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"The Cardioprotective Effect of Protocatechuic Acid on Myocardial Ischemia/Reperfusion Injury." Journal of Pharmacological Sciences 125.2 (2014): 176-183. WB ; Rat. PubMed:24942213 [IF=2.13] Cetintas, Vildan Bozok, et al. "Effects of flavopiridol on critical regulation pathways of CD133high/CD44high lung cancer stem cells." Medicine 95.43 (2016): e5150. IF(ICC) ; Mouse. PubMed:27787370 [IF=2.1] Zhang, Beiru, et al. "The Toxicity Mechanisms of Action of Aβ25–35 in Isolated Rat Cardiac Myocytes." Molecules 19.8 (2014): 12242-12257. WB ; Rat. PubMed:25123188 [IF=2.1] Hu, Hanhua, et al. "Drug-induced apoptosis of Echinococcus granulosus protoscoleces." Parasitology research 109.2 (2011): 453-459. IHC-P ; Others. PubMed:21365454 [IF=2.088] Wu LY, et al. Biochanin A Reduces Inflammatory Injury and Neuronal Apoptosis following Subarachnoid Hemorrhage via Suppression of the TLRs/TIRAP/MyD88/NF-B Pathway. Behav Neurol. 2018 Jun 3;2018:1960106. WB ; Rat. PubMed:29971136 [IF=2.03] Aksu, Ilkay, et al. "Anxiety correlates to decreased blood and prefrontal cortex IGF-1 levels in streptozotocin induced diabetes." Neuroscience Letters (2012). Rat. PubMed:23123774 [IF=1.949] Li Q et al. The effects of photobiomodulation therapy on mouse pre-osteoblast cell line MC3T3-E1 proliferation and apoptosis via miR-503/Wnt3a pathway.(2018) Lasers Med Sci. Sep 15 WB ; mouse. PubMed:30218348 [IF=1.894] An N et al. Involvement of Activation of Mitogen-Activated Protein Kinase (MAPK)/Extracellular Signal-Regulated Kinase (ERK) Signaling Pathway in Proliferation of Urethral Plate Fibroblasts in Finasteride-Induced Rat Hypospadias.(2018) Med Sci Monit. WB ; Rat . PubMed:30538214 [IF=1.871] Huang XW et al. Scutellarein suppresses Aβ?induced memory impairment via inhibition of the NF?κB pathway in vivo and in vitro. Oncol Lett. 2019 Jun;17(6):5581-5589. WB ; Rat. 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"The cytotoxic and pro-apoptotic effects of phenylephrine on corneal stromal cells via a mitochondrion-dependent pathway both in vitro and in vivo." Experimental and Toxicologic Pathology (2016). ELISA ; Human. PubMed:27344612 [IF=1.701] Kosutova P et al. Reduction of Lung Inflammation, Oxidative Stress and Apoptosis by the PDE4 Inhibitor Roflumilast in Experimental Model of Acute Lung Injury. Physiol Res. 2018 Dec 31;67(Supplementum 4):S645-S654. IHC ; Rabbit. PubMed:30607971 [IF=1.69] Song et al. Anti-autophagic and anti-apoptotic effects of memantine in a SH-SY5Y cell model of Alzheimer's disease via mammalian target of rapamycin-dependent and -independent pathways. (2015) Mol.Med.Re. 12:7615-22 WB ; Human. PubMed:26459718 [IF=1.64] Kosutova, P., et al. "Intravenous dexamethasone attenuated inflammation and influenced apoptosis of lung cells in an experimental model of acute lung injury." Physiological research 65.Supplementum 5 (2016): S663. IHC-P ; Rabbit. 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"8?bromo?7?methoxychrysin induces apoptosis by regulating Akt/FOXO3a pathway in cisplatin?sensitive and resistant ovarian cancer cells." Molecular medicine reports 12.4 (2015): 5100-5108. WB ; Human. PubMed:26151347 [IF=1.55] Han, Ning, et al. "Agmatine protects Müller cells from high?concentration glucose?induced cell damage via N?methyl?D?aspartic acid receptor inhibition." Molecular medicine reports 12.1 (2015): 1098-1106. WB ; Rat. PubMed:25816073 [IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082. WB ; Rat. PubMed:27665780 [IF=1.51] Sun, Y‐L., et al. "Relationship between apoptosis and proliferation in granulosa and theca cells of cystic follicles in sows." Reproduction in domestic animals 47.4 (2012): 601-608. IHC-P ; Pig. PubMed:22023076 [IF=1.48] Liu, Yong?Chao, et al. 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"Matrine inhibits diethylnitrosamine-induced HCC proliferation in rats through inducing apoptosis via p53, Bax-dependent caspase-3 activation pathway and down-regulating MLCK overexpression (Supplement 2016)." Iranian Journal of Pharmaceutical Research (2016). WB ; Rat. PubMed:27642320 [IF=.87] Ochigbo, Grace Onyeche, et al. "Polyphenol-rich fraction of Parquetina nigrescens mitigates dichlorvos-induced neurotoxicity and apoptosis." Journal of Ayurveda and Integrative Medicine (2017). Rat. PubMed:28256303 [IF=0] Li et al. Morusin suppresses breast cancer cell growth in vitro and in vivo through C/EBPβ and PPARγ mediated lipoapoptosis. (2015) J.Exp.Clin.Cancer.Res. 34:137 WB ; Human. PubMed:26538209 [IF=0] Wang, Yu, et al. "Tanshinone II A Relieves Adriamycin-induced Myocardial Injury in Rat Model." International Journal of Chemistry 8.1 (2016): 40. WB ; Rat. PubMed:10.5539/ijc.v8n1p40 [IF=0] Petty, Howard R. "NANOPARTICLE THERAPY IN CANCER." 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PubMed:30813721 研究领域肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡 Alzheimer's 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, 产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2μg/Test ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量28kDa细胞定位细胞浆 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human caspase-3 p17 subunit:1-100/277 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.Reacts with Caspase-3 subunit p17 and precursor.Function:Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.Subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce.Subcellular Location:Cytoplasm.Tissue Specificity:Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.Post-translational modifications:Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.Similarity:Belongs to the peptidase C14A family.SWISS:P42574Gene ID:836Database links:Entrez Gene: 836 HumanEntrez Gene: 12367 MouseEntrez Gene: 397244 PigEntrez Gene: 100008840 RabbitEntrez Gene: 25402 RatOmim: 600636 HumanSwissProt: P42574 HumanSwissProt: P70677 MouseSwissProt: Q95ND5 PigSwissProt: Q8MJC3 RabbitSwissProt: P55213 RatUnigene: 141125 HumanUnigene: 34405 MouseUnigene: 10562 RatImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.Caspase3广泛分布于各种不同类型的细胞中，是Caspase家族中最重要的凋亡执行者之一，激活的Caspase-3能使许多与细胞结构、细胞周期及DNA修复等相关蛋白或激酶失活，从而使细胞凋亡.产品图片Sample:Lane 1: Spleen (Mouse) Lysate at 40 ugLane 2: Lung (Mouse) Lysate at 40 ugLane 3: Lymph node (Mouse) Lysate at 40 ugLane 4: Cerebrum (Mouse) Lysate at 40 ugLane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ugLane 6: Spleen (Rat) Lysate at 40 ugLane 7: Lung (Rat) Lysate at 40 ugLane 8: Lymph node (Rat) Lysate at 40 ugLane 9: Cerebrum (Rat) Lysate at 40 ugLane 10: Hela (Human) Cell Lysate at 30 ugPrimary: Anti-Caspase-3 (bs-0081R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 35 kDObserved band size: 37 kDSample:Lane 1: Raji (Human) Cell Lysate at 30 ugLane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ugLane 3: Lung (Mouse) Lysate at 40 ugLane 4: Lung (Rat) Lysate at 40 ugPrimary: Anti-Caspase-3 (bs-0081R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 35 kDObserved band size: 37 kDSample:Kidney (Mouse) Lysate at 40 ugPrimary: Anti-Caspase-3 (bs-0081R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 28 kDObserved band size: 17 kDSample:Liver (Mouse) Lysate at 40 ugPrimary: Anti-Caspase-3 (bs-0081R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 28 kDObserved band size: 28 kDSample: Raji Cell lysate;Primary: Anti-Caspase-3 (bs-0081R) at 1:300;Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;Predicted band size: 17/28 kDObserved band size: 26 kDSample:Liver(Sheep)Lysate at 40 ugPrimary: Anti-Caspase-3(bs-0081R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 28kDObserved band size: 30kDSample:Lung(Mouse) Lysate at 40 ugHela(Human) Cell Lysate at 30 ugNIH/3T3(Mouse) Cell Lysate at 30 ugPrimary: Anti-Caspase-3 (bs-0081R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 35/29/19/17 kDObserved band size: 38 kDProtein:Lung(Mouse) lysate at 30ug;Primary: Anti-caspase-3 (bs-0081R) at 1:300 dilution;Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 5000;Predicted band size : 17/28 kDObserved band size : 28 kDTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Caspase-3 Polyclonal Antibody, Unconjugated(bs-0081R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: rabbit pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Caspase-3 Polyclonal Antibody, Unconjugated(bs-0081R) 1:300, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Caspase-3) polyclonal Antibody, Unconjugated (bs-0081R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.The figure annotation:The blue histogram is unstained cells. The Orange histogram is cells stained with Rabbit IgG/FITC (bs-0295P-FITC).The green histogram is cells stained with Rabbit Anti-Caspase-3/FITC Conjugated antibody (bs-0081R-FITC).ControlsPositive control: HepG 2 cellsIsotype control: Cell lines treated with Rabbit IgG/FITC (bs-0295P-FITC) instead of the primary antibody to confirm that primary antibody binding is specific. 2μg in 1 00μL1 X PBS containing 0.5% BSA.The figure annotation:The blue histogram is unstained cells.The Orange histogram is cells stained with Rabbit IgG/FITC (bs-0295P-FITC).The green histogram is cells stained with Rabbit Anti-Caspase-3/FITC Conjugated antibody (bs-0081R-FITC).ControlsPositive control: A549 cellsIsotype control: Cell lines treated with Rabbit IgG/FITC(bs-0295P-FITC) instead of the primary antibody to confirm that primary antibody binding is specific. 3μg in 1 00 μL 1 X PBS containing 0.5% BSA.Blank control:Mouse spleen.Primary Antibody (green line): Rabbit Anti-Caspase-3 antibody (bs-0081R)Dilution: 2μg /10^6 cells;Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody : Goat anti-rabbit IgG-AF488Dilution: 1μg /test.ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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